MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1937 440.0430 439.0357
1938 440.2368 439.2295
1939 440.3259 439.3186
1940 440.9753 439.9680
1941 441.0869 440.0796
1942 441.0870 440.0797
1943 441.1215 440.1142
1944 441.1225 440.1152
1945 441.2187 440.2114
1946 442.9429 441.9356
1947 443.0438 442.0365
1948 443.1026 442.0953
1949 443.1029 442.0956
1950 443.1030 442.0957
1951 443.2137 442.2064
1952 444.1097 443.1024
1953 445.0814 444.0741
1954 445.0822 444.0749
1955 445.1545 444.1472
1956 445.1652 444.1579
1958 446.2976 445.2903
1959 446.2979 445.2906
1960 447.0965 446.0892
1961 447.1338 446.1265
1962 449.2162 448.2089
1963 449.2181 448.2108
1964 449.2185 448.2112
1965 449.2196 448.2123
1966 450.1771 449.1698
1967 450.1780 449.1707
1968 450.2470 449.2397
1969 451.1684 450.1611
1970 451.1949 450.1876
1971 451.1957 450.1884
1972 451.1964 450.1891
1973 451.1964 450.1891
1974 451.1973 450.1900
1975 451.1973 450.1900
1976 451.1975 450.1902
1977 451.1975 450.1902
1978 451.2331 450.2258
1979 451.2333 450.2260
1980 451.2335 450.2262
1981 451.2335 450.2262
1982 451.2350 450.2277
1983 451.2357 450.2284
1984 451.2667 450.2594
1985 451.2687 450.2614
1986 452.0291 451.0218
1987 453.1231 452.1158
1988 453.1233 452.1160
1989 453.1725 452.1652
1990 453.1729 452.1656
1991 453.2098 452.2025
1992 453.2104 452.2031
1993 453.2105 452.2032
1994 453.2113 452.2040
1995 453.2116 452.2043
1996 453.2117 452.2044
1997 453.2117 452.2044
1998 453.2118 452.2045
1999 453.2128 452.2055
2000 453.2130 452.2057
2001 453.2131 452.2058
2002 453.2137 452.2064
2003 453.2481 452.2408
2004 453.2493 452.2420
2005 455.1012 454.0939
2006 455.1028 454.0955
2007 455.1032 454.0959
2008 455.1386 454.1313
2009 455.1906 454.1833
2010 455.1920 454.1847
2011 457.0826 456.0753
2012 457.1182 456.1109
2013 457.1681 456.1608
2014 459.0953 458.0880
2015 459.0981 458.0908
2016 459.0981 458.0908
2017 459.0982 458.0909
2018 459.1331 458.1258
2019 459.1334 458.1261
2020 459.2216 458.2143
2021 461.0769 460.0696
2022 461.1111 460.1038
2023 461.1148 460.1075
2024 463.0928 462.0855
2025 463.2096 462.2023
2026 463.2344 462.2271
2027 463.2672 462.2599
2028 465.1232 464.1159
2029 465.2130 464.2057
2030 465.2135 464.2062
2031 466.0312 465.0239
2032 466.0432 465.0359
2033 467.0694 466.0621
2034 467.2273 466.2200
2035 467.2290 466.2217
2036 467.2737 466.2664
2037 468.2675 467.2602
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