MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1836 425.1788 424.1715
1837 425.1795 424.1722
1838 425.1797 424.1724
1839 425.1798 424.1725
1840 425.1800 424.1727
1841 425.1801 424.1728
1842 425.1804 424.1731
1843 425.1808 424.1735
1844 425.2146 424.2073
1845 425.2148 424.2075
1846 425.2155 424.2082
1847 425.2169 424.2096
1848 425.2174 424.2101
1849 425.2188 424.2115
1850 427.0127 426.0054
1851 427.1073 426.1000
1852 427.1076 426.1003
1853 427.1578 426.1505
1854 427.1751 426.1678
1855 427.1961 426.1888
1856 427.1964 426.1891
1857 427.1965 426.1892
1858 427.1968 426.1895
1859 427.2353 426.2280
1860 429.0847 428.0774
1861 429.0870 428.0797
1862 429.0873 428.0800
1863 429.0874 428.0801
1864 429.1218 428.1145
1865 429.1229 428.1156
1866 429.1456 428.1383
1867 429.1546 428.1473
1868 429.1817 428.1744
1869 430.8566 429.8493
1870 431.0657 430.0584
1871 431.0661 430.0588
1872 431.1003 430.0930
1873 431.1019 430.0946
1874 431.1026 430.0953
1875 431.1393 430.1320
1876 432.2802 431.2729
1877 432.8854 431.8781
1878 433.1157 432.1084
1879 433.1849 432.1776
1880 433.1866 432.1793
1881 433.1866 432.1793
1883 435.0747 434.0674
1884 435.2002 434.1929
1885 435.2010 434.1937
1886 435.2011 434.1938
1887 435.2012 434.1939
1888 435.2012 434.1939
1889 435.2017 434.1944
1890 435.2020 434.1947
1891 435.2022 434.1949
1892 435.2024 434.1951
1893 435.2026 434.1953
1894 435.2033 434.1960
1895 435.2038 434.1965
1896 435.2045 434.1972
1897 436.0206 435.0133
1898 437.0041 435.9968
1899 437.0879 436.0806
1900 437.0896 436.0823
1901 437.1383 436.1310
1902 437.1793 436.1720
1903 437.1799 436.1726
1904 437.1805 436.1732
1905 437.1809 436.1736
1906 437.2150 436.2077
1907 437.2154 436.2081
1908 437.2156 436.2083
1909 437.2164 436.2091
1910 437.2164 436.2091
1911 437.2168 436.2095
1912 437.2168 436.2095
1913 437.2169 436.2096
1914 437.2174 436.2101
1915 437.2178 436.2105
1916 437.2178 436.2105
1917 437.2181 436.2108
1918 437.2191 436.2118
1919 437.2192 436.2119
1920 437.2197 436.2124
1921 437.2365 436.2292
1922 438.2296 437.2223
1923 439.1062 438.0989
1924 439.1941 438.1868
1925 439.1949 438.1876
1926 439.1965 438.1892
1927 439.1975 438.1902
1928 439.2310 438.2237
1929 439.2321 438.2248
1930 439.2322 438.2249
1931 439.2323 438.2250
1932 439.2325 438.2252
1933 439.2326 438.2253
1934 439.2326 438.2253
1935 439.2340 438.2267
1936 439.2345 438.2272
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