MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1736 411.2006 410.1933
1737 411.2008 410.1935
1738 411.2014 410.1941
1739 411.2014 410.1941
1740 413.0919 412.0846
1741 413.0921 412.0848
1742 413.0922 412.0849
1743 413.1407 412.1334
1744 415.0686 414.0613
1745 415.0708 414.0635
1746 415.0710 414.0637
1747 415.0933 414.0860
1748 415.1077 414.1004
1749 415.1187 414.1114
1750 415.1299 414.1226
1751 415.1416 414.1343
1752 415.1428 414.1355
1753 415.1564 414.1491
1754 416.2618 415.2545
1755 416.2975 415.2902
1756 416.3756 415.3683
1757 417.0509 416.0436
1758 417.0852 416.0779
1759 417.0859 416.0786
1760 417.0869 416.0796
1761 417.0870 416.0797
1762 417.1233 416.1160
1763 417.1234 416.1161
1764 417.1933 416.1860
1765 417.2062 416.1989
1766 418.0445 417.0372
1767 419.0650 418.0577
1768 419.1158 418.1085
1769 419.1714 418.1641
1770 419.2063 418.1990
1771 419.2067 418.1994
1772 419.2075 418.2002
1773 419.2076 418.2003
1774 419.2078 418.2005
1775 419.2083 418.2010
1776 419.2091 418.2018
1777 419.2093 418.2020
1778 419.2419 418.2346
1779 419.9890 418.9817
1780 420.1931 419.1858
1781 420.9350 419.9277
1782 421.0092 420.0019
1783 421.0611 420.0538
1784 421.1842 420.1769
1785 421.1847 420.1774
1786 421.1853 420.1780
1787 421.1854 420.1781
1788 421.1855 420.1782
1789 421.1858 420.1785
1790 421.1858 420.1785
1791 421.1864 420.1791
1792 421.1865 420.1792
1793 421.1874 420.1801
1794 421.1879 420.1806
1795 421.2203 420.2130
1796 421.2218 420.2145
1797 421.2220 420.2147
1798 421.2224 420.2151
1799 421.2228 420.2155
1800 421.2231 420.2158
1801 421.2234 420.2161
1802 421.2243 420.2170
1803 421.2244 420.2171
1804 421.2245 420.2172
1805 421.2249 420.2176
1806 421.2257 420.2184
1807 422.0046 420.9973
1808 422.0049 420.9976
1809 422.9302 421.9229
1810 423.1649 422.1576
1811 423.1651 422.1578
1812 423.1989 422.1916
1813 423.1995 422.1922
1814 423.1998 422.1925
1815 423.1998 422.1925
1816 423.2003 422.1930
1817 423.2004 422.1931
1818 423.2010 422.1937
1819 423.2012 422.1939
1820 423.2012 422.1939
1821 423.2013 422.1940
1822 423.2014 422.1941
1823 423.2020 422.1947
1824 423.2024 422.1951
1825 423.2025 422.1952
1826 423.2027 422.1954
1827 423.2034 422.1961
1828 423.2039 422.1966
1829 423.2064 422.1991
1830 423.2379 422.2306
1831 423.2386 422.2313
1832 423.2397 422.2324
1833 423.2400 422.2327
1834 425.0912 424.0839
1835 425.1284 424.1211
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