MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1635 399.2027 398.1954
1636 401.0548 400.0475
1637 401.0905 400.0832
1638 401.0916 400.0843
1639 401.0917 400.0844
1640 401.0919 400.0846
1641 401.0920 400.0847
1642 401.1041 400.0968
1643 401.1137 400.1064
1644 401.1280 400.1207
1645 401.1399 400.1326
1646 401.2882 400.2809
1647 401.9641 400.9568
1648 402.2477 401.2404
1649 403.0704 402.0631
1650 403.0704 402.0631
1651 403.1056 402.0983
1652 403.1073 402.1000
1653 403.1739 402.1666
1654 403.1763 402.1690
1655 403.1768 402.1695
1656 403.1772 402.1699
1658 403.2322 402.2249
1659 405.0498 404.0425
1660 405.1005 404.0932
1661 405.1890 404.1817
1662 405.1896 404.1823
1663 405.1903 404.1830
1664 405.1907 404.1834
1665 405.1916 404.1843
1666 405.1918 404.1845
1667 405.1922 404.1849
1668 405.1922 404.1849
1669 405.1931 404.1858
1670 405.1933 404.1860
1671 405.1933 404.1860
1672 405.2279 404.2206
1673 406.0093 405.0020
1674 406.9940 405.9867
1675 407.1151 406.1078
1676 407.1687 406.1614
1677 407.1689 406.1616
1678 407.1691 406.1618
1679 407.1691 406.1618
1680 407.1693 406.1620
1681 407.1698 406.1625
1682 407.1700 406.1627
1683 407.1708 406.1635
1684 407.1710 406.1637
1685 407.1716 406.1643
1686 407.2052 406.1979
1687 407.2058 406.1985
1688 407.2059 406.1986
1689 407.2060 406.1987
1690 407.2060 406.1987
1691 407.2066 406.1993
1692 407.2070 406.1997
1693 407.2071 406.1998
1694 407.2080 406.2007
1695 407.2087 406.2014
1696 407.2087 406.2014
1697 407.2088 406.2015
1698 407.2088 406.2015
1699 409.0092 408.0019
1700 409.0967 408.0894
1701 409.1636 408.1563
1702 409.1666 408.1593
1703 409.1671 408.1598
1704 409.1834 408.1761
1705 409.1835 408.1762
1706 409.1838 408.1765
1707 409.1844 408.1771
1708 409.1848 408.1775
1709 409.1848 408.1775
1710 409.1848 408.1775
1711 409.1849 408.1776
1712 409.1852 408.1779
1713 409.1853 408.1780
1714 409.1861 408.1788
1715 409.1861 408.1788
1716 409.1875 408.1802
1717 409.2206 408.2133
1718 409.2213 408.2140
1719 409.2233 408.2160
1720 409.2238 408.2165
1721 409.2249 408.2176
1722 411.0140 410.0067
1723 411.0760 410.0687
1724 411.0765 410.0692
1725 411.1122 410.1049
1726 411.1624 410.1551
1727 411.1632 410.1559
1728 411.1641 410.1568
1729 411.1642 410.1569
1730 411.1644 410.1571
1731 411.1649 410.1576
1732 411.1999 410.1926
1733 411.2003 410.1930
1734 411.2003 410.1930
1735 411.2005 410.1932
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