MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4336)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1535 393.2982 392.2909
1536 393.2983 392.2910
1537 393.2985 392.2912
1538 393.2985 392.2912
1539 393.2986 392.2913
1540 393.2987 392.2914
1541 393.2988 392.2915
1542 393.2988 392.2915
1543 393.2991 392.2918
1544 393.2991 392.2918
1545 393.2991 392.2918
1546 393.2991 392.2918
1547 393.2993 392.2920
1548 393.2994 392.2921
1549 393.2995 392.2922
1550 393.2995 392.2922
1551 393.2995 392.2922
1552 393.2996 392.2923
1553 393.2996 392.2923
1554 393.2997 392.2924
1555 393.2997 392.2924
1556 393.3001 392.2928
1557 393.3001 392.2928
1558 393.3002 392.2929
1559 393.3006 392.2933
1560 393.3009 392.2936
1561 393.3010 392.2937
1562 393.3012 392.2939
1563 393.3018 392.2945
1564 393.3021 392.2948
1565 393.3028 392.2955
1566 393.8818 392.8745
1567 394.0096 393.0023
1568 395.0753 394.0680
1569 395.1158 394.1085
1570 395.1679 394.1606
1571 395.1682 394.1609
1572 395.1683 394.1610
1573 395.1685 394.1612
1574 395.1685 394.1612
1575 395.1688 394.1615
1576 395.1689 394.1616
1577 395.1694 394.1621
1578 395.1695 394.1622
1579 395.1696 394.1623
1580 395.1697 394.1624
1581 395.1698 394.1625
1582 395.1700 394.1627
1583 395.1701 394.1628
1584 395.1702 394.1629
1585 395.1704 394.1631
1586 395.1708 394.1635
1587 395.1712 394.1639
1588 395.2040 394.1967
1589 395.2042 394.1969
1590 395.2043 394.1970
1591 395.2047 394.1974
1592 395.2050 394.1977
1593 395.2052 394.1979
1594 395.2061 394.1988
1595 395.2067 394.1994
1596 395.2072 394.1999
1597 395.2084 394.2011
1598 395.2088 394.2015
1599 397.0466 396.0393
1600 397.0511 396.0438
1601 397.0962 396.0889
1602 397.1465 396.1392
1603 397.1467 396.1394
1604 397.1507 396.1434
1605 397.1835 396.1762
1606 397.1840 396.1767
1607 397.1840 396.1767
1608 397.1844 396.1771
1609 397.1846 396.1773
1610 397.1849 396.1776
1611 397.1850 396.1777
1612 397.1855 396.1782
1613 397.1855 396.1782
1614 397.1858 396.1785
1615 397.1865 396.1792
1616 397.1865 396.1792
1617 397.1867 396.1794
1618 397.1869 396.1796
1619 397.2215 396.2142
1620 397.2218 396.2145
1621 397.2219 396.2146
1622 397.2219 396.2146
1623 397.2222 396.2149
1624 397.2240 396.2167
1625 398.1405 397.1332
1626 399.0742 398.0669
1627 399.0760 398.0687
1628 399.0765 398.0692
1629 399.1122 398.1049
1630 399.1129 398.1056
1631 399.1264 398.1191
1632 399.1473 398.1400
1633 399.1482 398.1409
1634 399.1625 398.1552
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