MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1435 381.1924 380.1851
1436 381.1926 380.1853
1437 381.9311 380.9238
1438 383.0809 382.0736
1439 383.0812 382.0739
1440 383.1174 382.1101
1441 383.1310 382.1237
1442 383.1317 382.1244
1443 383.1332 382.1259
1444 383.1682 382.1609
1445 383.1691 382.1618
1446 383.1694 382.1621
1447 383.1694 382.1621
1448 383.1698 382.1625
1449 383.1699 382.1626
1450 383.1700 382.1627
1451 383.1700 382.1627
1452 383.1701 382.1628
1453 383.1703 382.1630
1454 383.1704 382.1631
1455 383.2058 382.1985
1456 385.0596 384.0523
1457 385.0603 384.0530
1458 385.0965 384.0892
1459 385.0966 384.0893
1460 385.1281 384.1208
1461 385.1479 384.1406
1462 385.1484 384.1411
1463 385.1661 384.1588
1464 385.2212 384.2139
1465 387.0734 386.0661
1466 387.0758 386.0685
1467 387.0760 386.0687
1468 387.0761 386.0688
1469 387.0999 386.0926
1470 387.1123 386.1050
1471 387.1124 386.1051
1472 387.1240 386.1167
1473 387.1371 386.1298
1474 387.1815 386.1742
1475 387.1825 386.1752
1476 388.2551 387.2478
1477 389.0545 388.0472
1478 389.0553 388.0480
1479 389.0916 388.0843
1480 389.1032 388.0959
1481 389.2036 388.1963
1482 389.2185 388.2112
1483 389.2536 388.2463
1484 391.0702 390.0629
1485 391.1077 390.1004
1486 391.1735 390.1662
1487 391.1747 390.1674
1488 391.1747 390.1674
1489 391.1748 390.1675
1490 391.1750 390.1677
1491 391.1754 390.1681
1492 391.1763 390.1690
1493 391.1763 390.1690
1494 391.1773 390.1700
1495 391.2122 390.2049
1496 391.2131 390.2058
1497 391.2132 390.2059
1498 391.2137 390.2064
1499 391.2138 390.2065
1500 391.2139 390.2066
1501 391.2390 390.2317
1502 391.2861 390.2788
1503 392.1714 391.1641
1504 392.1721 391.1648
1505 392.1724 391.1651
1506 393.1176 392.1103
1507 393.1525 392.1452
1508 393.1533 392.1460
1509 393.1534 392.1461
1510 393.1534 392.1461
1511 393.1540 392.1467
1512 393.1541 392.1468
1513 393.1543 392.1470
1514 393.1550 392.1477
1515 393.1552 392.1479
1516 393.1891 392.1818
1517 393.1891 392.1818
1518 393.1898 392.1825
1519 393.1901 392.1828
1520 393.1901 392.1828
1521 393.1903 392.1830
1522 393.1904 392.1831
1523 393.1904 392.1831
1524 393.1905 392.1832
1525 393.1916 392.1843
1526 393.1917 392.1844
1527 393.1918 392.1845
1528 393.1919 392.1846
1529 393.1924 392.1851
1530 393.1932 392.1859
1531 393.1936 392.1863
1532 393.2104 392.2031
1533 393.2978 392.2905
1534 393.2980 392.2907
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