MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1335 375.8642 374.8569
1336 375.9987 374.9914
1337 376.2343 375.2270
1338 376.2345 375.2272
1339 376.9828 375.9755
1340 377.1577 376.1504
1341 377.1578 376.1505
1342 377.1581 376.1508
1343 377.1582 376.1509
1344 377.1582 376.1509
1345 377.1584 376.1511
1346 377.1588 376.1515
1347 377.1589 376.1516
1348 377.1591 376.1518
1349 377.1596 376.1523
1350 377.1597 376.1524
1351 377.1599 376.1526
1352 377.1601 376.1528
1353 377.1607 376.1534
1354 377.1950 376.1877
1355 377.1952 376.1879
1356 377.1965 376.1892
1357 377.1967 376.1894
1358 377.1967 376.1894
1359 377.1967 376.1894
1360 377.1972 376.1899
1361 377.1974 376.1901
1362 377.1977 376.1904
1363 377.1979 376.1906
1364 377.9781 376.9708
1365 378.0242 377.0169
1366 378.9856 377.9783
1367 379.1374 378.1301
1368 379.1375 378.1302
1369 379.1379 378.1306
1370 379.1381 378.1308
1371 379.1382 378.1309
1372 379.1383 378.1310
1373 379.1384 378.1311
1374 379.1386 378.1313
1375 379.1732 378.1659
1376 379.1733 378.1660
1377 379.1735 378.1662
1378 379.1735 378.1662
1379 379.1738 378.1665
1380 379.1738 378.1665
1381 379.1739 378.1666
1382 379.1739 378.1666
1383 379.1740 378.1667
1384 379.1744 378.1671
1385 379.1746 378.1673
1386 379.1746 378.1673
1387 379.1747 378.1674
1388 379.1750 378.1677
1389 379.1754 378.1681
1390 379.1754 378.1681
1391 379.1765 378.1692
1392 379.1767 378.1694
1393 379.1775 378.1702
1394 379.2099 378.2026
1395 379.2116 378.2043
1396 379.2120 378.2047
1397 379.2120 378.2047
1398 379.2124 378.2051
1399 379.2127 378.2054
1400 379.2129 378.2056
1401 379.2133 378.2060
1402 379.2138 378.2065
1403 381.0642 380.0569
1404 381.1348 380.1275
1405 381.1379 380.1306
1406 381.1517 380.1444
1407 381.1525 380.1452
1408 381.1534 380.1461
1409 381.1536 380.1463
1410 381.1537 380.1464
1411 381.1538 380.1465
1412 381.1542 380.1469
1413 381.1542 380.1469
1414 381.1543 380.1470
1415 381.1548 380.1475
1416 381.1549 380.1476
1417 381.1890 380.1817
1418 381.1891 380.1818
1419 381.1892 380.1819
1420 381.1893 380.1820
1421 381.1893 380.1820
1422 381.1895 380.1822
1423 381.1899 380.1826
1424 381.1899 380.1826
1425 381.1902 380.1829
1426 381.1903 380.1830
1427 381.1903 380.1830
1428 381.1903 380.1830
1429 381.1904 380.1831
1430 381.1905 380.1832
1431 381.1905 380.1832
1432 381.1918 380.1845
1433 381.1919 380.1846
1434 381.1924 380.1851
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