MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4336)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1035 355.1193 354.1120
1036 355.1377 354.1304
1037 355.1378 354.1305
1038 355.1380 354.1307
1039 355.1381 354.1308
1040 355.1382 354.1309
1041 355.1384 354.1311
1042 355.1384 354.1311
1043 355.1387 354.1314
1044 355.1388 354.1315
1045 355.1395 354.1322
1046 355.1727 354.1654
1047 355.1737 354.1664
1048 355.1742 354.1669
1049 355.1746 354.1673
1050 355.1749 354.1676
1051 355.1750 354.1677
1052 355.1751 354.1678
1053 355.9095 354.9022
1054 356.0443 355.0370
1055 357.0284 356.0211
1056 357.0634 356.0561
1057 357.0645 356.0572
1058 357.0652 356.0579
1059 357.0652 356.0579
1060 357.0656 356.0583
1061 357.0876 356.0803
1062 357.0980 356.0907
1063 357.1124 356.1051
1064 357.1138 356.1065
1065 357.1317 356.1244
1066 357.1337 356.1264
1067 357.1528 356.1455
1068 357.1537 356.1464
1069 357.1542 356.1469
1070 357.1555 356.1482
1071 357.1898 356.1825
1072 359.0427 358.0354
1073 359.0441 358.0368
1074 359.0807 358.0734
1075 359.0810 358.0737
1076 359.0938 358.0865
1077 359.1042 358.0969
1078 359.1102 358.1029
1079 359.1116 358.1043
1080 359.1481 358.1408
1081 359.1483 358.1410
1082 359.1484 358.1411
1083 359.1491 358.1418
1084 359.1497 358.1424
1085 359.1503 358.1430
1086 359.2311 358.2238
1087 359.2424 358.2351
1088 359.9537 358.9464
1089 360.2034 359.1961
1090 360.2153 359.2080
1091 360.2404 359.2331
1092 360.9317 359.9244
1093 361.0106 360.0033
1094 361.0233 360.0160
1095 361.0596 360.0523
1096 361.0602 360.0529
1097 361.0968 360.0895
1098 361.1631 360.1558
1099 361.1638 360.1565
1100 361.1639 360.1566
1101 361.1640 360.1567
1102 361.1642 360.1569
1103 361.1644 360.1571
1104 361.1646 360.1573
1105 361.1650 360.1577
1106 361.1650 360.1577
1107 361.1654 360.1581
1108 361.1661 360.1588
1109 361.1663 360.1590
1110 361.2013 360.1940
1111 361.2019 360.1946
1112 362.0698 361.0625
1113 362.1776 361.1703
1114 362.9978 361.9905
1115 363.0391 362.0318
1116 363.1039 362.0966
1117 363.1424 362.1351
1118 363.1424 362.1351
1119 363.1425 362.1352
1120 363.1426 362.1353
1121 363.1427 362.1354
1122 363.1428 362.1355
1123 363.1430 362.1357
1124 363.1430 362.1357
1125 363.1437 362.1364
1126 363.1438 362.1365
1127 363.1440 362.1367
1128 363.1441 362.1368
1129 363.1443 362.1370
1130 363.1783 362.1710
1131 363.1786 362.1713
1132 363.1790 362.1717
1133 363.1791 362.1718
1134 363.1793 362.1720
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