MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
835 342.0407 341.0334
836 342.0654 341.0581
837 342.0656 341.0583
838 342.9139 341.9066
839 343.0490 342.0417
840 343.0490 342.0417
841 343.0493 342.0420
842 343.0495 342.0422
843 343.0502 342.0429
844 343.0858 342.0785
845 343.0988 342.0915
846 343.1167 342.1094
847 343.1552 342.1479
848 344.2080 343.2007
849 344.2292 343.2219
850 345.0285 344.0212
851 345.0288 344.0215
852 345.0649 344.0576
853 345.0655 344.0582
854 345.0655 344.0582
855 345.0880 344.0807
856 345.1019 344.0946
857 345.1136 344.1063
858 345.1138 344.1065
859 345.1327 344.1254
860 345.1332 344.1259
861 345.1333 344.1260
862 345.1345 344.1272
863 345.1348 344.1275
864 345.1551 344.1478
865 345.1710 344.1637
866 345.2264 344.2191
867 345.9289 344.9216
868 347.0215 346.0142
869 347.0439 346.0366
870 347.0807 346.0734
871 347.1475 346.1402
872 347.1476 346.1403
873 347.1480 346.1407
874 347.1480 346.1407
875 347.1480 346.1407
876 347.1481 346.1408
877 347.1482 346.1409
878 347.1483 346.1410
879 347.1485 346.1412
880 347.1485 346.1412
881 347.1485 346.1412
882 347.1487 346.1414
883 347.1490 346.1417
884 347.1497 346.1424
885 347.1503 346.1430
886 347.1862 346.1789
887 347.1865 346.1792
888 347.1868 346.1795
889 347.1868 346.1795
890 347.1869 346.1796
891 347.1873 346.1800
892 348.0043 346.9970
893 348.0530 347.0457
894 348.0538 347.0465
895 348.0759 347.0686
896 348.2019 347.1946
897 348.2029 347.1956
898 349.0309 348.0236
899 349.0733 348.0660
900 349.1257 348.1184
901 349.1268 348.1195
902 349.1273 348.1200
903 349.1273 348.1200
904 349.1273 348.1200
905 349.1274 348.1201
906 349.1277 348.1204
907 349.1293 348.1220
908 349.1455 348.1382
909 349.1626 348.1553
910 349.1627 348.1554
911 349.1628 348.1555
912 349.1630 348.1557
913 349.1631 348.1558
914 349.1632 348.1559
915 349.1635 348.1562
916 349.1635 348.1562
917 349.1638 348.1565
918 349.1639 348.1566
919 349.1640 348.1567
920 349.1643 348.1570
921 349.1645 348.1572
922 349.1646 348.1573
923 349.1650 348.1577
924 349.1651 348.1578
925 349.1653 348.1580
926 349.1657 348.1584
927 349.1661 348.1588
928 349.1661 348.1588
929 349.1767 348.1694
930 349.1847 348.1774
931 349.2003 348.1930
932 349.2014 348.1941
933 349.2018 348.1945
934 349.2019 348.1946
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