MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
735 337.1272 336.1199
736 337.1277 336.1204
737 337.1277 336.1204
738 337.1279 336.1206
739 337.1280 336.1207
740 337.1280 336.1207
741 337.1282 336.1209
742 337.1330 336.1257
743 337.1626 336.1553
744 337.1628 336.1555
745 337.1629 336.1556
746 337.1629 336.1556
747 337.1630 336.1557
748 337.1630 336.1557
749 337.1634 336.1561
750 337.1634 336.1561
751 337.1636 336.1563
752 337.1636 336.1563
753 337.1638 336.1565
754 337.1639 336.1566
755 337.1639 336.1566
756 337.1642 336.1569
757 337.1643 336.1570
758 337.1647 336.1574
759 337.1648 336.1575
760 337.1654 336.1581
761 337.1657 336.1584
762 337.1663 336.1590
763 338.0568 337.0495
764 338.0694 337.0621
765 338.9607 337.9534
766 338.9879 337.9806
767 339.0095 338.0022
768 339.0209 338.0136
769 339.0547 338.0474
770 339.0770 338.0697
771 339.1046 338.0973
772 339.1060 338.0987
773 339.1062 338.0989
774 339.1064 338.0991
775 339.1071 338.0998
776 339.1076 338.1003
777 339.1133 338.1060
778 339.1219 338.1146
779 339.1220 338.1147
780 339.1419 338.1346
781 339.1419 338.1346
782 339.1420 338.1347
783 339.1422 338.1349
784 339.1424 338.1351
785 339.1426 338.1353
786 339.1427 338.1354
787 339.1430 338.1357
788 339.1432 338.1359
789 339.1432 338.1359
790 339.1433 338.1360
791 339.1433 338.1360
792 339.1433 338.1360
793 339.1434 338.1361
794 339.1438 338.1365
795 339.1591 338.1518
796 339.1782 338.1709
797 339.1788 338.1715
798 339.1788 338.1715
799 339.1788 338.1715
800 339.1790 338.1717
801 339.1797 338.1724
802 339.1810 338.1737
803 340.0497 339.0424
804 340.0499 339.0426
805 340.9843 339.9770
806 341.0706 340.0633
807 341.0707 340.0634
808 341.0922 340.0849
809 341.1021 340.0948
810 341.1064 340.0991
811 341.1204 340.1131
812 341.1213 340.1140
813 341.1215 340.1142
814 341.1216 340.1143
815 341.1222 340.1149
816 341.1225 340.1152
817 341.1226 340.1153
818 341.1227 340.1154
819 341.1389 340.1316
820 341.1390 340.1317
821 341.1395 340.1322
822 341.1579 340.1506
823 341.1582 340.1509
824 341.1584 340.1511
825 341.1585 340.1512
826 341.1586 340.1513
827 341.1591 340.1518
828 341.1591 340.1518
829 341.1591 340.1518
830 341.1592 340.1519
831 341.1592 340.1519
832 341.1957 340.1884
833 341.1992 340.1919
834 342.0287 341.0214
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