MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
635 333.0285 332.0212
636 333.0542 332.0469
637 333.0561 332.0488
638 333.1310 332.1237
639 333.1318 332.1245
640 333.1319 332.1246
641 333.1321 332.1248
642 333.1322 332.1249
643 333.1322 332.1249
644 333.1323 332.1250
645 333.1324 332.1251
646 333.1328 332.1255
647 333.1339 332.1266
648 333.1356 332.1283
649 333.1683 332.1610
650 333.1685 332.1612
651 333.1688 332.1615
652 333.1690 332.1617
653 333.1691 332.1618
654 333.1694 332.1621
655 333.1694 332.1621
656 333.1695 332.1622
657 333.1698 332.1625
658 333.1700 332.1627
659 333.1706 332.1633
660 333.1707 332.1634
661 333.1708 332.1635
662 333.1899 332.1826
663 333.2135 332.2062
664 333.2143 332.2070
665 333.2146 332.2073
666 333.2149 332.2076
667 334.0092 333.0019
668 334.0372 333.0299
669 335.0217 334.0144
670 335.0324 334.0251
671 335.0733 334.0660
672 335.0898 334.0825
673 335.0899 334.0826
674 335.0957 334.0884
675 335.1101 334.1028
676 335.1104 334.1031
677 335.1108 334.1035
678 335.1113 334.1040
679 335.1118 334.1045
680 335.1121 334.1048
681 335.1126 334.1053
682 335.1174 334.1101
683 335.1473 334.1400
684 335.1474 334.1401
685 335.1476 334.1403
686 335.1477 334.1404
687 335.1478 334.1405
688 335.1479 334.1406
689 335.1480 334.1407
690 335.1482 334.1409
691 335.1483 334.1410
692 335.1484 334.1411
693 335.1486 334.1413
694 335.1486 334.1413
695 335.1487 334.1414
696 335.1487 334.1414
697 335.1488 334.1415
698 335.1495 334.1422
699 335.1499 334.1426
700 335.1502 334.1429
701 335.1843 334.1770
702 335.1846 334.1773
703 335.1848 334.1775
704 335.1855 334.1782
705 335.1855 334.1782
706 335.1858 334.1785
707 335.1862 334.1789
708 335.1864 334.1791
709 335.1866 334.1793
710 335.1866 334.1793
711 335.1867 334.1794
712 335.1873 334.1800
713 335.1985 334.1912
714 335.9659 334.9586
715 335.9673 334.9600
716 335.9796 334.9723
717 336.0527 335.0454
718 337.0335 336.0262
719 337.0336 336.0263
720 337.0359 336.0286
721 337.0741 336.0668
722 337.0881 336.0808
723 337.0886 336.0813
724 337.0988 336.0915
725 337.1064 336.0991
726 337.1073 336.1000
727 337.1259 336.1186
728 337.1262 336.1189
729 337.1263 336.1190
730 337.1266 336.1193
731 337.1266 336.1193
732 337.1270 336.1197
733 337.1270 336.1197
734 337.1271 336.1198
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