MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4336)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
535 325.1651 324.1578
536 325.1652 324.1579
537 325.1655 324.1582
538 325.1660 324.1587
539 325.2006 324.1933
540 326.0340 325.0267
541 326.0697 325.0624
542 326.0703 325.0630
543 326.1980 325.1907
544 326.8937 325.8864
545 327.0182 326.0109
546 327.0540 326.0467
547 327.0542 326.0469
548 327.0543 326.0470
549 327.0543 326.0470
550 327.0543 326.0470
551 327.0544 326.0471
552 327.0544 326.0471
553 327.0761 326.0688
554 327.0766 326.0693
555 327.0860 326.0787
556 327.0866 326.0793
557 327.0901 326.0828
558 327.0902 326.0829
559 327.1028 326.0955
560 327.1032 326.0959
561 327.1058 326.0985
562 327.1060 326.0987
563 327.1062 326.0989
564 327.1067 326.0994
565 327.1069 326.0996
566 327.1143 326.1070
567 327.1209 326.1136
568 327.1217 326.1144
569 327.1220 326.1147
570 327.1230 326.1157
571 327.1429 326.1356
572 327.1429 326.1356
573 327.1434 326.1361
574 327.1435 326.1362
575 327.1435 326.1362
576 327.1436 326.1363
577 327.1444 326.1371
578 327.1447 326.1374
579 327.1785 326.1712
580 327.1786 326.1713
581 327.1786 326.1713
582 327.1789 326.1716
583 327.1793 326.1720
584 327.2023 326.1950
585 327.9635 326.9562
586 328.0180 327.0107
587 328.0495 327.0422
588 328.0496 327.0423
589 329.0325 328.0252
590 329.0329 328.0256
591 329.0332 328.0259
592 329.0334 328.0261
593 329.0336 328.0263
594 329.0341 328.0268
595 329.0343 328.0270
596 329.0685 328.0612
597 329.0690 328.0617
598 329.0695 328.0622
599 329.0696 328.0623
600 329.0828 328.0755
601 329.0829 328.0756
602 329.1389 328.1316
603 329.1575 328.1502
604 329.1584 328.1511
605 329.1587 328.1514
606 329.1592 328.1519
607 329.1596 328.1523
608 329.1598 328.1525
609 329.1948 328.1875
610 330.2307 329.2234
611 330.9217 329.9144
612 331.0496 330.0423
613 331.0498 330.0425
614 331.0499 330.0426
615 331.0859 330.0786
616 331.0864 330.0791
617 331.1158 330.1085
618 331.1168 330.1095
619 331.1174 330.1101
620 331.1536 330.1463
621 331.1540 330.1467
622 331.1541 330.1468
623 331.1544 330.1471
624 331.1544 330.1471
625 331.1552 330.1479
626 331.1757 330.1684
627 331.1759 330.1686
628 331.1759 330.1686
629 331.2110 330.2037
630 331.6977 330.6904
631 332.1722 331.1649
632 332.2083 331.2010
633 332.2086 331.2013
634 332.9007 331.8934
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