MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
435 321.1694 320.1621
436 321.1695 320.1622
437 321.1698 320.1625
438 321.1699 320.1626
439 321.1699 320.1626
440 321.1701 320.1628
441 321.1702 320.1629
442 321.1706 320.1633
443 321.2066 320.1993
444 322.0392 321.0319
445 322.1777 321.1704
446 322.8988 321.8915
447 322.9832 321.9759
448 322.9834 321.9761
449 322.9835 321.9762
450 323.0180 322.0107
451 323.0592 322.0519
452 323.0717 322.0644
453 323.1107 322.1034
454 323.1111 322.1038
455 323.1115 322.1042
456 323.1116 322.1043
457 323.1117 322.1044
458 323.1117 322.1044
459 323.1118 322.1045
460 323.1118 322.1045
461 323.1119 322.1046
462 323.1122 322.1049
463 323.1122 322.1049
464 323.1127 322.1054
465 323.1287 322.1214
466 323.1468 322.1395
467 323.1469 322.1396
468 323.1472 322.1399
469 323.1474 322.1401
470 323.1474 322.1401
471 323.1476 322.1403
472 323.1476 322.1403
473 323.1478 322.1405
474 323.1479 322.1406
475 323.1481 322.1408
476 323.1481 322.1408
477 323.1482 322.1409
478 323.1483 322.1410
479 323.1483 322.1410
480 323.1484 322.1411
481 323.1484 322.1411
482 323.1485 322.1412
483 323.1489 322.1416
484 323.1489 322.1416
485 323.1493 322.1420
486 323.1496 322.1423
487 323.1841 322.1768
488 323.1846 322.1773
489 323.1846 322.1773
490 323.1846 322.1773
491 323.1851 322.1778
492 323.1858 322.1785
493 323.1863 322.1790
494 323.1868 322.1795
495 323.1873 322.1800
496 323.9675 322.9602
497 324.0548 323.0475
498 324.9515 323.9442
499 325.0387 324.0314
500 325.0611 324.0538
501 325.0739 324.0666
502 325.0869 324.0796
503 325.0890 324.0817
504 325.0900 324.0827
505 325.0907 324.0834
506 325.1047 324.0974
507 325.1049 324.0976
508 325.1069 324.0996
509 325.1110 324.1037
510 325.1117 324.1044
511 325.1259 324.1186
512 325.1266 324.1193
513 325.1266 324.1193
514 325.1270 324.1197
515 325.1271 324.1198
516 325.1272 324.1199
517 325.1273 324.1200
518 325.1273 324.1200
519 325.1273 324.1200
520 325.1275 324.1202
521 325.1276 324.1203
522 325.1277 324.1204
523 325.1278 324.1205
524 325.1278 324.1205
525 325.1284 324.1211
526 325.1629 324.1556
527 325.1631 324.1558
528 325.1631 324.1558
529 325.1638 324.1565
530 325.1638 324.1565
531 325.1639 324.1566
532 325.1640 324.1567
533 325.1642 324.1569
534 325.1642 324.1569
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