MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
335 315.9271 314.9198
336 316.1532 315.1459
337 316.1762 315.1689
338 316.1765 315.1692
339 316.1765 315.1692
340 317.0324 316.0251
341 317.0336 316.0263
342 317.0338 316.0265
343 317.0696 316.0623
344 317.0827 316.0754
345 317.1002 316.0929
346 317.1002 316.0929
347 317.1013 316.0940
348 317.1036 316.0963
349 317.1071 316.0998
350 317.1374 316.1301
351 317.1375 316.1302
352 317.1383 316.1310
353 317.1391 316.1318
354 317.1750 316.1677
355 317.1750 316.1677
356 317.1758 316.1685
357 317.1761 316.1688
358 317.1766 316.1693
359 317.1772 316.1699
360 318.1563 317.1490
361 318.1914 317.1841
362 318.1918 317.1845
363 318.1922 317.1849
364 318.1924 317.1851
365 318.1926 317.1853
366 318.1927 317.1854
367 319.0125 318.0052
368 319.0408 318.0335
369 319.0494 318.0421
370 319.0496 318.0423
371 319.0960 318.0887
372 319.1157 318.1084
373 319.1161 318.1088
374 319.1161 318.1088
375 319.1162 318.1089
376 319.1163 318.1090
377 319.1165 318.1092
378 319.1166 318.1093
379 319.1166 318.1093
380 319.1167 318.1094
381 319.1169 318.1096
382 319.1171 318.1098
383 319.1522 318.1449
384 319.1530 318.1457
385 319.1531 318.1458
386 319.1534 318.1461
387 319.1535 318.1462
388 319.1536 318.1463
389 319.1539 318.1466
390 319.1541 318.1468
391 319.1544 318.1471
392 319.1546 318.1473
393 319.1546 318.1473
394 319.1548 318.1475
395 319.1553 318.1480
396 319.1656 318.1583
397 319.1887 318.1814
398 319.1906 318.1833
399 319.9937 318.9864
400 320.0577 319.0504
401 320.0593 319.0520
402 320.1721 319.1648
403 320.9677 319.9604
404 321.0432 320.0359
405 321.0774 320.0701
406 321.0946 320.0873
407 321.0963 320.0890
408 321.0963 320.0890
409 321.0965 320.0892
410 321.1310 320.1237
411 321.1312 320.1239
412 321.1313 320.1240
413 321.1317 320.1244
414 321.1318 320.1245
415 321.1319 320.1246
416 321.1320 320.1247
417 321.1320 320.1247
418 321.1321 320.1248
419 321.1324 320.1251
420 321.1324 320.1251
421 321.1325 320.1252
422 321.1325 320.1252
423 321.1325 320.1252
424 321.1327 320.1254
425 321.1328 320.1255
426 321.1328 320.1255
427 321.1342 320.1269
428 321.1505 320.1432
429 321.1683 320.1610
430 321.1686 320.1613
431 321.1689 320.1616
432 321.1690 320.1617
433 321.1691 320.1618
434 321.1691 320.1618
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