MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
235 311.1110 310.1037
236 311.1111 310.1038
237 311.1112 310.1039
238 311.1112 310.1039
239 311.1113 310.1040
240 311.1115 310.1042
241 311.1115 310.1042
242 311.1115 310.1042
243 311.1120 310.1047
244 311.1473 310.1400
245 311.1476 310.1403
246 311.1476 310.1403
247 311.1476 310.1403
248 311.1477 310.1404
249 311.1478 310.1405
250 311.1479 310.1406
251 311.1480 310.1407
252 311.1482 310.1409
253 311.1482 310.1409
254 311.1483 310.1410
255 311.1484 310.1411
256 311.1488 310.1415
257 311.1490 310.1417
258 311.1494 310.1421
259 311.1497 310.1424
260 311.1830 310.1757
261 311.1836 310.1763
262 311.1850 310.1777
263 311.1855 310.1782
264 311.1869 310.1796
265 311.1872 310.1799
266 312.0181 311.0108
267 312.0182 311.0109
268 312.0544 311.0471
269 312.0547 311.0474
270 312.0914 311.0841
271 313.0374 312.0301
272 313.0380 312.0307
273 313.0381 312.0308
274 313.0384 312.0311
275 313.0390 312.0317
276 313.0610 312.0537
277 313.0613 312.0540
278 313.0750 312.0677
279 313.0867 312.0794
280 313.0875 312.0802
281 313.0898 312.0825
282 313.0898 312.0825
283 313.0969 312.0896
284 313.0975 312.0902
285 313.1061 312.0988
286 313.1073 312.1000
287 313.1078 312.1005
288 313.1268 312.1195
289 313.1270 312.1197
290 313.1271 312.1198
291 313.1280 312.1207
292 313.1280 312.1207
293 313.1304 312.1231
294 313.1648 312.1575
295 313.1651 312.1578
296 313.1655 312.1582
297 314.0333 313.0260
298 314.0466 313.0393
299 314.1974 313.1901
300 315.0168 314.0095
301 315.0179 314.0106
302 315.0181 314.0108
303 315.0516 314.0443
304 315.0530 314.0457
305 315.0538 314.0465
306 315.0540 314.0467
307 315.0540 314.0467
308 315.0541 314.0468
309 315.0543 314.0470
310 315.0544 314.0471
311 315.0669 314.0596
312 315.0671 314.0598
313 315.1020 314.0947
314 315.1137 314.1064
315 315.1208 314.1135
316 315.1209 314.1136
317 315.1214 314.1141
318 315.1220 314.1147
319 315.1222 314.1149
320 315.1224 314.1151
321 315.1428 314.1355
322 315.1429 314.1356
323 315.1433 314.1360
324 315.1433 314.1360
325 315.1442 314.1369
326 315.1450 314.1377
327 315.1452 314.1379
328 315.1456 314.1383
329 315.1591 314.1518
330 315.1598 314.1525
331 315.1608 314.1535
332 315.1790 314.1717
333 315.1809 314.1736
334 315.1814 314.1741
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