MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
135 307.1159 306.1086
136 307.1161 306.1088
137 307.1162 306.1089
138 307.1163 306.1090
139 307.1164 306.1091
140 307.1166 306.1093
141 307.1167 306.1094
142 307.1167 306.1094
143 307.1170 306.1097
144 307.1170 306.1097
145 307.1173 306.1100
146 307.1522 306.1449
147 307.1525 306.1452
148 307.1525 306.1452
149 307.1526 306.1453
150 307.1527 306.1454
151 307.1527 306.1454
152 307.1530 306.1457
153 307.1530 306.1457
154 307.1531 306.1458
155 307.1531 306.1458
156 307.1537 306.1464
157 307.1539 306.1466
158 307.1540 306.1467
159 307.1540 306.1467
160 307.1544 306.1471
161 307.1545 306.1472
162 307.1548 306.1475
163 307.1910 306.1837
164 307.1915 306.1842
165 307.9711 306.9638
166 308.0233 307.0160
167 308.1492 307.1419
168 308.1498 307.1425
169 309.0427 308.0354
170 309.0429 308.0356
171 309.0805 308.0732
172 309.0947 308.0874
173 309.0948 308.0875
174 309.0956 308.0883
175 309.0961 308.0888
176 309.0963 308.0890
177 309.0964 308.0891
178 309.1104 308.1031
179 309.1313 308.1240
180 309.1313 308.1240
181 309.1315 308.1242
182 309.1316 308.1243
183 309.1317 308.1244
184 309.1317 308.1244
185 309.1318 308.1245
186 309.1319 308.1246
187 309.1322 308.1249
188 309.1323 308.1250
189 309.1323 308.1250
190 309.1323 308.1250
191 309.1324 308.1251
192 309.1324 308.1251
193 309.1325 308.1252
194 309.1327 308.1254
195 309.1331 308.1258
196 309.1332 308.1259
197 309.1334 308.1261
198 309.1337 308.1264
199 309.1490 308.1417
200 309.1677 308.1604
201 309.1680 308.1607
202 309.1681 308.1608
203 309.1681 308.1608
204 309.1683 308.1610
205 309.1685 308.1612
206 309.1688 308.1615
207 309.1688 308.1615
208 309.1689 308.1616
209 309.1689 308.1616
210 309.1690 308.1617
211 309.1690 308.1617
212 309.1690 308.1617
213 309.1693 308.1620
214 309.1695 308.1622
215 309.1695 308.1622
216 309.1700 308.1627
217 309.1701 308.1628
218 309.1704 308.1631
219 310.0381 309.0308
220 310.0386 309.0313
221 310.0390 309.0317
222 310.0731 309.0658
223 310.1124 309.1051
224 311.0230 310.0157
225 311.0233 310.0160
226 311.0591 310.0518
227 311.0592 310.0519
228 311.0721 310.0648
229 311.0729 310.0656
230 311.0814 310.0741
231 311.0893 310.0820
232 311.0905 310.0832
233 311.0916 310.0843
234 311.1109 310.1036
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