MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
35 301.9117 300.9044
36 301.9117 300.9044
37 301.9476 300.9403
38 302.1616 301.1543
39 302.1971 301.1898
40 302.1976 301.1903
41 302.1980 301.1907
42 302.8701 301.8628
43 302.8702 301.8629
44 303.0164 302.0091
45 303.0176 302.0103
46 303.0179 302.0106
47 303.0541 302.0468
48 303.0542 302.0469
49 303.0670 302.0597
50 303.0671 302.0598
51 303.0848 302.0775
52 303.0851 302.0778
53 303.0854 302.0781
54 303.1031 302.0958
55 303.1205 302.1132
56 303.1213 302.1140
57 303.1214 302.1141
58 303.1215 302.1142
59 303.1215 302.1142
60 303.1217 302.1144
61 303.1220 302.1147
62 303.1221 302.1148
63 303.1221 302.1148
64 303.1223 302.1150
65 303.1228 302.1155
66 303.1587 302.1514
67 303.1588 302.1515
68 303.1590 302.1517
69 303.1593 302.1520
70 303.1595 302.1522
71 303.1727 302.1654
72 303.1786 302.1713
73 303.1790 302.1717
74 303.1794 302.1721
75 303.9636 302.9563
76 304.0645 303.0572
77 304.0662 303.0589
78 304.1405 303.1332
79 304.1755 303.1682
80 304.1767 303.1694
81 304.1772 303.1699
82 304.9715 303.9642
83 305.0331 304.0258
84 305.0493 304.0420
85 305.0703 304.0630
86 305.0988 304.0915
87 305.1002 304.0929
88 305.1005 304.0932
89 305.1006 304.0933
90 305.1008 304.0935
91 305.1009 304.0936
92 305.1010 304.0937
93 305.1010 304.0937
94 305.1036 304.0963
95 305.1362 304.1289
96 305.1364 304.1291
97 305.1364 304.1291
98 305.1366 304.1293
99 305.1367 304.1294
100 305.1367 304.1294
101 305.1367 304.1294
102 305.1374 304.1301
103 305.1374 304.1301
104 305.1375 304.1302
105 305.1375 304.1302
106 305.1378 304.1305
107 305.1379 304.1306
108 305.1382 304.1309
109 305.1382 304.1309
110 305.1386 304.1313
111 305.1387 304.1314
112 305.1389 304.1316
113 305.1392 304.1319
114 305.1753 304.1680
115 305.9567 304.9494
116 305.9569 304.9496
117 306.0060 304.9987
118 306.1558 305.1485
119 306.1560 305.1487
120 306.1564 305.1491
121 306.1565 305.1492
122 306.1566 305.1493
123 306.1567 305.1494
124 306.9879 305.9806
125 307.0197 306.0124
126 307.0272 306.0199
127 307.0641 306.0568
128 307.0771 306.0698
129 307.0805 306.0732
130 307.0949 306.0876
131 307.0963 306.0890
132 307.1150 306.1077
133 307.1155 306.1082
134 307.1159 306.1086
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