MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4336)

Page: 1 2 3 4 5 6 … 44 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2430 444.2361 886.4576 886.4872 -33.4 0 23 0.061 +1Score > 23 indicates identity LAAGQGLTR + Deamidated (NQ)
2317 353.6823 705.3500 705.3479 3.00 0 22 0.063 +1Score > 22 indicates identity ALEAMR + Oxidation (M)
2269 651.3366 650.3293 650.3388 -14.5 0 21 0.056 +1Score > 21 indicates identity VYDVR
2587 513.8076 1025.6006 1025.6121 -11.1 0 20 0.054 +1Score > 20 indicates identity ALGLNPTVIK + Deamidated (NQ)
2194 589.3670 588.3597 588.3595 0.39 0 20 0.056 +1Score > 20 indicates identity LTSIR
2506 961.5394 960.5321 960.5393 -7.43 0 20 0.087 +1Score > 22 indicates identity VLQAFVQR + Deamidated (NQ)
3070 495.6016 1483.7830 1483.7379 30.4 1 20 0.078 +1Score > 22 indicates identity
Score > 21 indicates homology		 STRHLALTDQGQR + 2 Deamidated (NQ)
2379 787.4730 786.4657 786.4599 7.37 0 19 0.12 +1Score > 22 indicates identity LAVLSER
2376 393.2480 784.4814 784.5171 -45.4 0 18 0.084 +1Score > 20 indicates identity VVLVISR
2229 309.6733 617.3320 617.3133 30.4 0 18 0.24 +1Score > 24 indicates identity SDAVAR
2562 499.7872 997.5598 997.5920 -32.3 0 18 0.089 +1Score > 20 indicates identity INALVVVDR
2310 688.4050 687.3977 687.3915 9.02 0 18 0.2 +1Score > 23 indicates identity IVNSVR + Deamidated (NQ)
2446 457.7723 913.5300 913.5749 -49.1 0 18 0.15 +1Score > 22 indicates identity LLLWLTR
2410 421.2217 840.4288 840.4705 -49.5 0 18 0.076 +1Score > 19 indicates identity QLNQPLK + Deamidated (NQ)
3048 739.3370 1476.6594 1476.6878 -19.2 1 17 0.12 +1Score > 21 indicates identity ERMGETLDELER
2293 672.4080 671.4007 671.3755 37.6 0 17 0.22 +1Score > 23 indicates identity TWIPR
2656 543.8026 1085.5906 1085.5717 17.5 0 17 0.21 +1Score > 23 indicates identity IVEAIEQQR + Deamidated (NQ)
2367 780.3839 779.3766 779.3450 40.6 0 17 0.12 +1Score > 20 indicates identity LFDNDR + Deamidated (NQ)
2278 659.3774 658.3701 658.4014 -47.5 1 17 0.15 +1Score > 24 indicates identity
Score > 21 indicates homology		 LVDKGK
2561 499.2958 996.5770 996.5604 16.7 0 17 0.096 +1Score > 19 indicates identity ALAELPLDR
2417 428.7677 855.5208 855.5290 -9.52 1 17 0.12 +1Score > 20 indicates identity IANRLLR + Deamidated (NQ)
2316 706.3549 705.3476 705.3592 -16.4 1 17 0.19 +1Score > 22 indicates identity DRMIR + Oxidation (M)
2401 412.7424 823.4702 823.4664 4.65 1 16 0.077 +1Score > 18 indicates identity VAVNHRK + Deamidated (NQ)
2291 336.6786 671.3426 671.3715 -42.9 0 16 0.12 +1Score > 20 indicates identity LAGQQR
2270 651.3478 650.3405 650.3387 2.74 0 16 0.12 +1Score > 20 indicates identity AELYR
2445 914.5367 913.5294 913.5749 -49.8 0 16 0.23 +1Score > 22 indicates identity LLLWLTR
3397 990.4986 1978.9826 1979.0800 -49.2 1 16 0.28 +1Score > 23 indicates identity HTVRAAIDQLLTQGLVSR + 2 Deamidated (NQ)
2339 736.3904 735.3831 735.3473 48.8 0 16 0.22 +1Score > 22 indicates identity LAQEMK + Oxidation (M); Deamidated (NQ)
3208 531.5696 1591.6870 1591.7487 -38.8 0 16 0.057 +1Score > 20 indicates identity
Score > 16 indicates homology		 MVTGWGQSMEPTIR
2323 708.4010 707.3937 707.3854 11.8 0 16 0.16 +1Score > 21 indicates identity
Score > 20 indicates homology		 LFNVSK + Deamidated (NQ)
3123 507.9366 1520.7880 1520.7140 48.6 1 16 0.13 +1Score > 22 indicates identity
Score > 19 indicates homology		 NMTIEQQAGEVRK + Oxidation (M); 2 Deamidated (NQ)
2345 750.4034 749.3961 749.3742 29.3 0 16 0.2 +1Score > 21 indicates identity TMQVVR + Oxidation (M); Deamidated (NQ)
3209 796.8511 1591.6876 1591.7487 -38.3 0 15 0.064 +1Score > 20 indicates identity
Score > 16 indicates homology		 MVTGWGQSMEPTIR
3954 658.5793 2630.2881 2630.2653 8.66 0 15 0.3 +1Score > 22 indicates identity TDSHPTIDAADLPYHVVAFDYGVK
2935 681.3661 1360.7176 1360.7099 5.71 1 15 0.27 +1Score > 22 indicates identity
Score > 22 indicates homology		 FQQNGLLRAEGK + Deamidated (NQ)
2183 571.3598 570.3525 570.3489 6.29 0 15 0.057 +1Score > 15 indicates identity LSVPR
2121 515.3326 514.3253 514.3227 5.06 0 15 0.48 +1Score > 24 indicates identity
Score > 24 indicates homology		 LAGVR
3608 718.6873 2153.0401 2152.9912 22.7 0 15 0.12 +1Score > 23 indicates identity
Score > 18 indicates homology		 AEIDEVQQGNWPAENNPLK + 2 Deamidated (NQ)
2266 325.6682 649.3218 649.3217 0.18 0 14 0.23 +1Score > 21 indicates identity ALGMSR + Oxidation (M)
2344 743.4450 742.4377 742.4337 5.38 0 14 0.36 +1Score > 23 indicates identity
Score > 22 indicates homology		 GLTLSPR
3574 535.2739 2137.0665 2137.1313 -30.3 1 14 0.07 +1Score > 23 indicates identity
Score > 15 indicates homology		 QSAVLDCHKGITLALANAVR + Deamidated (NQ)
2366 390.6954 779.3762 779.3450 40.1 0 14 0.24 +1Score > 20 indicates identity LFDNDR + Deamidated (NQ)
2767 399.9134 1196.7184 1196.6989 16.2 1 14 0.12 +1Score > 17 indicates identity NQGGQLLARIK
2230 618.3398 617.3325 617.3319 1.00 0 14 0.51 +1Score > 24 indicates identity
Score > 23 indicates homology		 CALVR
3164 775.3983 1548.7820 1548.7532 18.6 1 14 0.19 +1Score > 22 indicates identity
Score > 19 indicates homology		 SGELAIADEFGRER
3226 804.8481 1607.6816 1607.7436 -38.5 0 14 0.2 +1Score > 19 indicates identity MVTGWGQSMEPTIR + Oxidation (M)
3138 765.3563 1528.6980 1528.7733 -49.2 0 14 0.28 +1Score > 21 indicates identity NVIQISNDLENLR + 2 Deamidated (NQ)
3184 784.3880 1566.7614 1566.7889 -17.5 0 14 0.44 +1Score > 23 indicates identity STSVIAQYTAALQGR + 2 Deamidated (NQ)
3589 714.7208 2141.1406 2141.1150 11.9 0 14 0.19 +1Score > 22 indicates identity
Score > 19 indicates homology		 LGCQVQLATQGAEALELLAR + Deamidated (NQ)
2742 589.8137 1177.6128 1177.6013 9.84 0 13 0.41 +1Score > 22 indicates identity LLDSSVQMIR + Oxidation (M); Deamidated (NQ)
2429 443.7444 885.4742 885.4780 -4.29 1 13 0.39 +1Score > 22 indicates identity
Score > 22 indicates homology		 RQGEQIR
3060 493.9558 1478.8456 1478.7803 44.2 1 13 0.17 +1Score > 18 indicates identity DPSYLLLKDMLR + Oxidation (M)
2625 531.3082 1060.6018 1060.5587 40.7 0 13 0.25 +1Score > 20 indicates identity MLLDPSLTR + Oxidation (M)
2378 786.4475 785.4402 785.4759 -45.4 1 13 0.45 +1Score > 22 indicates identity LLDAARK
3366 638.2920 1911.8542 1911.8772 -12.0 0 13 0.14 +1Score > 20 indicates identity
Score > 17 indicates homology		 MSQVTNTPYEALEVGQK + Oxidation (M); 2 Deamidated (NQ)
2624 531.2878 1060.5610 1060.5454 14.7 0 13 0.089 +1Score > 22 indicates identity
Score > 15 indicates homology		 FVGTPAGAWR
3008 723.8497 1445.6848 1445.6973 -8.59 0 13 0.34 +1Score > 22 indicates identity
Score > 21 indicates homology		 AQLQAFAPMQPAR + Oxidation (M); 2 Deamidated (NQ)
2686 559.3071 1116.5996 1116.5564 38.8 0 13 0.12 +1Score > 23 indicates identity
Score > 16 indicates homology		 LYTQAHVER + Deamidated (NQ)
3203 794.3887 1586.7628 1586.7841 -13.4 1 13 0.25 +1Score > 23 indicates identity
Score > 19 indicates homology		 QFVDYYAERAGIR
2714 573.8675 1145.7204 1145.7060 12.6 0 13 0.053 +1Score > 13 indicates identity QSLILAILFK + Deamidated (NQ)
3512 1056.5724 2111.1302 2111.2255 -45.1 1 13 0.096 +1Score > 21 indicates identity
Score > 15 indicates homology		 YVLQGGLKGLLAPTWQVLR
2640 537.3067 1072.5988 1072.5877 10.4 1 13 0.46 +1Score > 22 indicates identity KVNIDTDLR
3010 362.9228 1447.6621 1447.7307 -47.4 0 13 0.36 +1Score > 21 indicates identity TTAGPQQVDVIYR + Deamidated (NQ)
2281 664.3457 663.3384 663.3374 1.59 0 13 0.39 +1Score > 22 indicates identity
Score > 21 indicates homology		 LMSASR
2518 485.7903 969.5660 969.5971 -32.0 0 13 0.35 +1Score > 21 indicates identity LAGVLGALTR
3528 706.6892 2117.0458 2117.1368 -43.0 1 13 0.33 +1Score > 23 indicates identity
Score > 20 indicates homology		 KYLTLDGGLAIDNVLNELR + Deamidated (NQ)
2629 1066.4989 1065.4916 1065.5025 -10.3 1 12 0.4 +1Score > 21 indicates identity RLYPSDCR
2750 594.3268 1186.6390 1186.6306 7.15 1 12 0.65 +1Score > 23 indicates identity VLQARQDSIR + 2 Deamidated (NQ)
2145 533.3292 532.3219 532.2969 47.0 1 12 0.25 +1Score > 19 indicates identity NGKSK
4059 972.5427 2914.6063 2914.5804 8.89 1 12 0.25 +1Score > 19 indicates identity QILIIGAGFAGLWSALSAIRQLDLNGSK + 3 Deamidated (NQ)
2307 688.3685 687.3612 687.3915 -44.1 0 12 0.35 +1Score > 24 indicates identity
Score > 20 indicates homology		 LLDATR
2340 369.2137 736.4128 736.3868 35.4 0 12 0.21 +1Score > 18 indicates identity FSQSLR
2288 667.2997 666.2924 666.2643 42.2 0 12 0.15 +1Score > 17 indicates identity MTQDR + Oxidation (M); Deamidated (NQ)
3310 587.6447 1759.9123 1759.8628 28.1 0 12 0.19 +1Score > 23 indicates identity
Score > 18 indicates homology		 TSSEIAPWTLVEANDK
2122 516.3170 515.3097 515.3180 -16.0 1 12 1 +1Score > 25 indicates identity
Score > 25 indicates homology		 GKGVR
2249 317.6708 633.3270 633.3156 18.1 0 12 0.46 +1Score > 21 indicates identity MAGLDK
3863 629.5593 2514.2081 2514.2748 -26.5 1 12 0.13 +1Score > 23 indicates identity
Score > 16 indicates homology		 GLAGMQERVIALQGSLDITSQPGR + Oxidation (M); 2 Deamidated (NQ)
2286 334.1533 666.2920 666.2829 13.7 0 12 0.16 +1Score > 17 indicates identity MTPMR + 2 Oxidation (M)
2084 489.2440 488.2367 488.2343 5.00 0 12 0.58 +1Score > 22 indicates identity AAGNR + Deamidated (NQ)
2438 450.2257 898.4368 898.4800 -48.0 0 12 0.24 +1Score > 18 indicates identity ILEYYAK
2801 612.8010 1223.5874 1223.5856 1.48 0 12 0.45 +1Score > 21 indicates identity
Score > 21 indicates homology		 ANGVLVGAEMTF + Oxidation (M)
3283 852.4283 1702.8420 1702.7654 45.0 0 12 0.17 +1Score > 23 indicates identity
Score > 17 indicates homology		 LQMLNQAVAHGAQMR + 2 Oxidation (M); 4 Deamidated (NQ)
2860 423.9034 1268.6884 1268.6976 -7.26 0 12 0.1 +1Score > 22 indicates identity
Score > 15 indicates homology		 LPLTIEDALER
2355 384.1801 766.3456 766.3684 -29.6 0 12 0.31 +1Score > 19 indicates identity FGGEVMK
2433 891.4106 890.4033 890.3916 13.2 0 12 0.35 +1Score > 20 indicates identity NAGEVMNR + Deamidated (NQ)
2680 1115.5250 1114.5177 1114.5692 -46.2 0 12 0.27 +1Score > 20 indicates identity
Score > 19 indicates homology		 LMVIGQDDPK
3046 369.9281 1475.6833 1475.7038 -13.9 0 12 0.57 +1Score > 22 indicates identity GSIELTQVAGQGCR + Deamidated (NQ)
2745 592.8210 1183.6274 1183.6309 -2.93 1 12 0.45 +1Score > 22 indicates identity
Score > 21 indicates homology		 TPRQGIDLQR + Deamidated (NQ)
3284 852.8833 1703.7520 1703.7850 -19.3 1 12 0.33 +1Score > 19 indicates identity VEGKDDETGEDLIQR + Deamidated (NQ)
2335 361.6991 721.3836 721.3680 21.7 0 12 0.4 +1Score > 20 indicates identity
Score > 20 indicates homology		 VISMEK + Oxidation (M)
2580 510.8024 1019.5902 1019.6352 -44.1 1 12 0.28 +1Score > 19 indicates identity LIGRVGHIR
2352 761.3567 760.3494 760.3715 -29.0 0 12 0.42 +1Score > 21 indicates identity
Score > 20 indicates homology		 QSLQER + Deamidated (NQ)
2382 397.7425 793.4704 793.4922 -27.5 1 12 0.17 +1Score > 16 indicates identity HGLKALR
2240 624.2803 623.2730 623.3027 -47.7 0 12 0.14 +1Score > 16 indicates identity GSTFGR
3306 880.4611 1758.9076 1758.9073 0.19 0 11 0.091 +1Score > 23 indicates identity
Score > 14 indicates homology		 MLAESVDSLITIPNEK
3511 704.7170 2111.1292 2111.2255 -45.6 1 11 0.15 +1Score > 21 indicates identity
Score > 16 indicates homology		 YVLQGGLKGLLAPTWQVLR
2137 527.3336 526.3263 526.3227 6.87 0 11 0.12 -1Score > 15 indicates identity IPAAR
3005 482.8944 1445.6614 1445.7303 -47.7 0 11 0.1 +1Score > 21 indicates identity
Score > 14 indicates homology		 LSVFNYAHLPER + Deamidated (NQ)
3009 723.8505 1445.6864 1445.7449 -40.4 1 11 0.62 +1Score > 22 indicates identity QLIERWMQAVR + Oxidation (M); Deamidated (NQ)
2287 667.2994 666.2921 666.2829 13.8 0 11 0.19 +1Score > 17 indicates identity MTPMR + 2 Oxidation (M)
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