MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 36)


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+1

Accession Score Description
1 Q88JG7_PSEPK 5946 Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1

+2

Accession Score Description
1 3FLAG_TEV_PP_2683 1692 Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683

+3

Accession Score Description
1 TRYP_PIG 212 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q88HN7_PSEPK 173 Chaperone-associated ATPase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3316 PE=4 SV=1

+5

Accession Score Description
1 Q88QY6_PSEPK 50 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0348 PE=4 SV=1

+6

Accession Score Description
1 CH60_PSEPK 47 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

-7

Accession Score Description
1 Q88FW8_PSEPK 42 Voltage-gated chloride channel family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3959 PE=4 SV=1
Score Mass Matches Sequences emPAI
7.1 Q88FW8_PSEPK 42 47841 4 (3) 1 (1) 0.14
Voltage-gated chloride channel family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3959 PE=4 SV=1

+4 peptide matches (2 non-duplicate, 2 duplicate)


+8

Accession Score Description
1 Q88H31_PSEPK 37 description

+9

Accession Score Description
1 Q88RB6_PSEPK 36 Fatty acid desaturase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0217 PE=4 SV=1

+10

Accession Score Description
1 Q88MB8_PSEPK 34 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
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