MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–36 (out of 36)

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+31

Accession Score Description
1 LOLD_PSEPK 18 Lipoprotein-releasing system ATP-binding protein LolD OS=Pseudomonas putida (strain KT2440) GN=lolD PE=3 SV=2

+32

Accession Score Description
1 Q88L70_PSEPK 17 Multidrug efflux RND transporter OS=Pseudomonas putida (strain KT2440) GN=PP_2065 PE=4 SV=1

+33

Accession Score Description
1 Q88JP4_PSEPK 16 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2605 PE=4 SV=1

-34

Accession Score Description
1 Q9X4K7_STRCH 15 Acyl-CoA carboxylase subunit beta OS=Streptomyces coelicolor OX=1902 GN=pccB PE=1 SV=1
Score Mass Matches Sequences emPAI
34.1 Q9X4K7_STRCH 15 57352 3 (1) 3 (1) 0.06
Acyl-CoA carboxylase subunit beta OS=Streptomyces coelicolor OX=1902 GN=pccB PE=1 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2344   743.4450 742.4377 742.4449 -9.73 1 2 5.3 +6Score > 23 indicates identity
Score > 22 indicates homology		 U K.LADLRR.R
2414   425.7852 849.5558 849.5297 30.8 1 15 0.028 +1Score > 13 indicates identity U R.HIVRGLR.Q
3510   704.3679 2110.0819 2110.0106 33.8 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 U R.LIQEYEDALLNPYTAAER.G + 2 Deamidated (NQ)

+35

Accession Score Description
1 Q88P97_PSEPK 15 Lipopolysaccharide export system protein LptC OS=Pseudomonas putida (strain KT2440) GN=lptC PE=3 SV=1

+36

Accession Score Description
1 ARCA_PSEPK 15 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1
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