MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (36 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 Q88JG7_PSEPK 5946 41896 204 (179) 35 (34) 935.41 Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1
2 3FLAG_TEV_PP_2683 1692 66327 60 (53) 37 (35) 11.33 Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683
3 TRYP_PIG 212 25078 9 (7) 4 (4) 1.40 Trypsin OS=Sus scrofa PE=1 SV=1
4 Q88HN7_PSEPK 173 103878 2 (2) 2 (2) 0.06 Chaperone-associated ATPase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3316 PE=4 SV=1
5 Q88QY6_PSEPK 50 105576 4 (2) 3 (1) 0.06 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0348 PE=4 SV=1
6 CH60_PSEPK 47 56765 2 (1) 2 (1) 0.06 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
7 Q88FW8_PSEPK 42 47841 4 (3) 1 (1) 0.14 Voltage-gated chloride channel family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3959 PE=4 SV=1
8 Q88H31_PSEPK 37 0 6 (4) 2 (2) 0.34 description
9 Q88RB6_PSEPK 36 45461 3 (1) 2 (1) 0.07 Fatty acid desaturase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0217 PE=4 SV=1
10 Q88MB8_PSEPK 34 84014 5 (1) 3 (1) 0.04 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
11 Q88H50_PSEPK 33 75115 2 (2) 1 (1) 0.04 Hydantoin utilization protein A OS=Pseudomonas putida (strain KT2440) GN=hyuA PE=4 SV=1
12 Q88M80_PSEPK 32 0 5 (2) 4 (2) 0.12 description
13 NICB_PSEPK 32 128479 1 (1) 1 (1) 0.03 Nicotinate dehydrogenase subunit B OS=Pseudomonas putida (strain KT2440) GN=nicB PE=1 SV=1
14 Q88N36_PSEPK 31 28810 2 (1) 1 (1) 0.12 3-oxoadipate enol-lactone hydrolase OS=Pseudomonas putida (strain KT2440) GN=pcaD PE=4 SV=1
15 Q88NE1_PSEPK 27 0 3 (2) 2 (1) 0.06 description
16 Q88GZ2_PSEPK 27 48613 1 (1) 1 (1) 0.07 Monooxygenase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3573 PE=4 SV=1
17 Q7CCB3_PSEPK 26 32633 3 (2) 1 (1) 0.21 Transcriptional regulator, LysR family OS=Pseudomonas putida (strain KT2440) GN=PP_4494 PE=4 SV=1
18 Q88J30_PSEPK 26 50514 4 (1) 3 (1) 0.07 Outer membrane protein OprJ OS=Pseudomonas putida (strain KT2440) GN=oprJ PE=3 SV=1
19 Q88KU6_PSEPK 25 89794 2 (1) 2 (1) 0.04 Outer membrane ferric siderophore receptor OS=Pseudomonas putida (strain KT2440) GN=PP_2193 PE=3 SV=1
20 Q88P58_PSEPK 23 0 1 (1) 1 (1) 0.14 description
21 Q88IK9_PSEPK 23 0 2 (1) 2 (1) 0.19 description
22 Q88ER3_PSEPK 23 15851 1 (1) 1 (1) 0.21 Flagellar basal-body rod protein FlgC OS=Pseudomonas putida (strain KT2440) GN=flgC PE=4 SV=1
23 Q88NY9_PSEPK 22 41265 1 (1) 1 (1) 0.08 Hydrolase, alpha/beta fold family OS=Pseudomonas putida (strain KT2440) GN=PP_1064 PE=4 SV=1
24 Q88ED3_PSEPK 22 48562 2 (1) 2 (1) 0.07 ATP-dependent RNA helicase, DEAD box family OS=Pseudomonas putida (strain KT2440) GN=PP_4532 PE=3 SV=1
25 Q88LN2_PSEPK 21 0 2 (1) 2 (1) 0.04 description
26 Q88NI1_PSEPK 21 73820 4 (2) 3 (1) 0.04 Methyl-accepting chemotaxis transducer OS=Pseudomonas putida (strain KT2440) GN=PP_1228 PE=4 SV=1
27 Q88F56_PSEPK 20 477994 4 (1) 4 (1) 0.01 Pyoverdine synthetase OS=Pseudomonas putida (strain KT2440) GN=PP_4243 PE=4 SV=1
28 Q88LF0_PSEPK 20 128606 2 (1) 2 (1) 0.03 Sensory box protein OS=Pseudomonas putida (strain KT2440) GN=PP_1983 PE=4 SV=1
29 Q88MU8_PSEPK 19 0 2 (1) 2 (1) 0.07 description
30 Q88E41_PSEPK 18 32388 1 (1) 1 (1) 0.10 Transcriptional regulator, LysR family OS=Pseudomonas putida (strain KT2440) GN=PP_4627 PE=4 SV=1
31 LOLD_PSEPK 18 24812 2 (1) 1 (1) 0.13 Lipoprotein-releasing system ATP-binding protein LolD OS=Pseudomonas putida (strain KT2440) GN=lolD PE=3 SV=2
32 Q88L70_PSEPK 17 110407 2 (1) 2 (1) 0.03 Multidrug efflux RND transporter OS=Pseudomonas putida (strain KT2440) GN=PP_2065 PE=4 SV=1
33 Q88JP4_PSEPK 16 60812 2 (1) 2 (1) 0.05 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2605 PE=4 SV=1
34 Q9X4K7_STRCH 15 57352 3 (1) 3 (1) 0.06 Acyl-CoA carboxylase subunit beta OS=Streptomyces coelicolor OX=1902 GN=pccB PE=1 SV=1
35 Q88P97_PSEPK 15 21336 2 (1) 1 (1) 0.16 Lipopolysaccharide export system protein LptC OS=Pseudomonas putida (strain KT2440) GN=lptC PE=3 SV=1
36 ARCA_PSEPK 15 46775 1 (1) 1 (1) 0.07 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

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