MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 36)

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+1

Accession Score Description
1 Q88JG7_PSEPK 5946 Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1

-2

Accession Score Description
1 3FLAG_TEV_PP_2683 1692 Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683
Score Mass Matches Sequences emPAI
2.1 3FLAG_TEV_PP_2683 1692 66327 60 (53) 37 (35) 11.33
Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683
3 samesets of 3FLAG_TEV_PP_2683
6HIS_TEV_PP_2683 1692 64438 60 (53) 37 (35) 12.26
Fusion_protein_N_term_6HISTEV_in_frame_with_PP_2683
Q88JG6_PSEPK 1692 62885 60 (53) 37 (35) 13.12
Sensory box histidine kinase/response regulator OS=Pseudomonas putida (strain KT2440) GN=PP_2683 PE=4 SV=1
JBx_206305 1692 66500 60 (53) 37 (35) 11.28
alt_start_1

-60 peptide matches (58 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2229   309.6733 617.3320 617.3285 5.69 0 16 0.36 +3Score > 24 indicates identity U R.SWLGR.G
2230   618.3398 617.3325 617.3285 6.46 0 10 1.2 +2Score > 24 indicates identity
Score > 23 indicates homology		 U R.SWLGR.G
2235   621.3724 620.3651 620.3646 0.88 0 21 0.023 +1Score > 17 indicates identity U K.ILGYR.I
2236   311.1901 620.3656 620.3646 1.72 0 21 0.021 +1Score > 17 indicates identity U K.ILGYR.I
2259   321.7188 641.4230 641.4224 0.97 0 34 0.0009 +1Score > 16 indicates identity U R.ILLGAR.R
2260   642.4304 641.4231 641.4224 1.09 0 24 0.0098 +1Score > 16 indicates identity U R.ILLGAR.R
2276   330.1786 658.3426 658.3398 4.28 0 39 0.0011 +1Score > 22 indicates identity U R.GIAQDR.L
2370   392.2295 782.4444 782.4398 5.88 0 46 8.6e-005 +1Score > 18 indicates identity U R.AANPALAR.M
2396   815.5359 814.5286 814.5276 1.24 0 33 0.0013 +1Score > 17 indicates identity U R.LLISTLR.E
2397   408.2716 814.5286 814.5276 1.26 0 43 0.00013 +1Score > 17 indicates identity U R.LLISTLR.E
2487   946.4870 945.4797 945.4767 3.20 0 26 0.022 +1Score > 23 indicates identity
Score > 22 indicates homology		 U R.TDELLEAR.D
2488   473.7479 945.4812 945.4767 4.81 0 52 7.2e-005 +1Score > 23 indicates identity U R.TDELLEAR.D
2525   487.7460 973.4774 973.4716 6.00 0 47 0.00022 +1Score > 23 indicates identity U R.LDELEAER.N
2527   488.2480 974.4814 974.4821 -0.72 0 42 0.00072 +1Score > 23 indicates identity U R.LEVWDTGR.G
2536   491.2347 980.4548 980.4498 5.17 0 42 0.00043 +1Score > 20 indicates identity U R.NFLSNACR.Y
2590   514.3008 1026.5870 1026.5822 4.75 0 64 2.6e-006 +1Score > 20 indicates identity U R.EGALLALQGR.A
2621   353.8477 1058.5213 1058.5145 6.42 0 26 0.022 +1Score > 22 indicates identity
Score > 22 indicates homology		 U K.SHYPELSAR.L
2622   530.2684 1058.5222 1058.5145 7.34 0 35 0.0017 +1Score > 22 indicates identity
Score > 20 indicates homology		 U K.SHYPELSAR.L
2653   542.3322 1082.6498 1082.6448 4.67 0 76 9e-008 +1Score > 18 indicates identity U K.GVGLGLAIVER.I
2660   546.8454 1091.6762 1091.6703 5.47 0 59 1.3e-006 +1Score > 13 indicates identity U R.AVLSQLLLVH.-
2749   396.5451 1186.6135 1186.6094 3.40 1 38 0.0019 +1Score > 23 indicates identity U R.KSHYPELSAR.L
2788   404.5895 1210.7467 1210.7397 5.72 1 60 2.1e-006 +1Score > 16 indicates identity U R.KGVGLGLAIVER.I
2789   606.3807 1210.7468 1210.7397 5.87 1 94 7.1e-010 +1Score > 15 indicates identity U R.KGVGLGLAIVER.I
2805   410.1961 1227.5665 1227.5632 2.64 0 51 4.1e-005 +1Score > 20 indicates identity U R.EHSLHGYETR.L
2806   614.7914 1227.5682 1227.5632 4.09 0 28 0.0092 +1Score > 20 indicates identity U R.EHSLHGYETR.L
2810   616.8521 1231.6896 1231.6812 6.82 0 66 2.1e-006 +1Score > 22 indicates identity U R.GSVFSIEVPLGK.E
2817 +1 619.3292 1236.6438 1236.6350 7.16 0 70 7.8e-007 +1Score > 22 indicates identity U R.IADYAISTDLR.L
2821   622.8193 1243.6240 1243.6156 6.77 1 21 0.061 +1Score > 23 indicates identity
Score > 21 indicates homology		 U R.LDELEAERNR.Y
2822   415.5488 1243.6246 1243.6156 7.19 1 22 0.038 +1Score > 22 indicates identity
Score > 21 indicates homology		 U R.LDELEAERNR.Y
2927   675.8808 1349.7470 1349.7415 4.09 0 72 3.3e-007 +1Score > 20 indicates identity
Score > 20 indicates homology		 U R.ALAGLLGLGDHSAR.K
2928   450.9230 1349.7472 1349.7415 4.18 0 59 7.2e-006 +1Score > 20 indicates identity U R.ALAGLLGLGDHSAR.K
2945   693.3565 1384.6984 1384.6946 2.76 0 66 2.7e-006 +1Score > 23 indicates identity U R.LQQLNDELEQR.V
2946   462.5739 1384.6999 1384.6946 3.79 0 41 0.00072 +1Score > 23 indicates identity U R.LQQLNDELEQR.V
2986   358.7332 1430.9037 1430.8973 4.47 0 42 6.6e-005 +1Score > 13 indicates identity U K.LGAPLLNKPVKPGK.L
2987   477.9755 1430.9047 1430.8973 5.16 0 45 3.2e-005 +1Score > 13 indicates identity U K.LGAPLLNKPVKPGK.L
3058   370.4684 1477.8445 1477.8365 5.42 1 28 0.0062 +1Score > 19 indicates identity U R.ALAGLLGLGDHSARK.S
3059   493.6224 1477.8454 1477.8365 6.01 1 47 8.2e-005 +1Score > 18 indicates identity U R.ALAGLLGLGDHSARK.S
3160   772.9276 1543.8406 1543.8358 3.12 0 97 1.7e-009 +1Score > 22 indicates identity U R.LDQAAVKPDVAVYR.L
3161   515.6214 1543.8424 1543.8358 4.24 0 85 1.4e-008 +1Score > 22 indicates identity
Score > 19 indicates homology		 U R.LDQAAVKPDVAVYR.L
D:\Xcalibur\Data\Jennifer\PRT1487 Andy\Andy2682a.raw

Score > 22 indicates identity

Score > 16 indicates homology
3273   419.2311 1672.8953 1672.8896 3.39 0 50 2.3e-005 -1Score > 22 indicates identity
Score > 16 indicates homology		 U R.ERPLPEAEHVLVER.T
0.96 1 0 2 2 YFQVTPGKHELVVR   + Deamidated (NQ)
3274   558.6392 1672.8958 1672.8896 3.67 0 56 2.4e-005 +1Score > 22 indicates identity U R.ERPLPEAEHVLVER.T
3289   862.9731 1723.9316 1723.9257 3.45 0 51 4.7e-005 +1Score > 21 indicates identity
Score > 20 indicates homology		 U R.EHFATAIPAVIITADR.S
3331   602.9877 1805.9413 1805.9312 5.58 0 81 6.6e-008 +1Score > 22 indicates identity
Score > 22 indicates homology		 U R.LQDIFLEFNQLDVGR.A
3332   904.4782 1806.9418 1806.9152 14.7 0 72 5.5e-007 +1Score > 22 indicates identity U R.LQDIFLEFNQLDVGR.A + Deamidated (NQ)
3337   609.0001 1823.9785 1823.9529 14.0 0 48 7.1e-005 +1Score > 21 indicates identity
Score > 19 indicates homology		 U K.YLAAASHDLLQPLNAAR.L + Deamidated (NQ)
3338   912.9967 1823.9788 1823.9529 14.2 0 64 2.5e-006 +1Score > 21 indicates identity U K.YLAAASHDLLQPLNAAR.L + Deamidated (NQ)
3445   517.7817 2067.0977 2067.0748 11.1 1 19 0.092 +1Score > 21 indicates identity U K.DKYLAAASHDLLQPLNAAR.L + Deamidated (NQ)
3446   690.0403 2067.0991 2067.0748 11.7 1 24 0.029 +1Score > 21 indicates identity U K.DKYLAAASHDLLQPLNAAR.L + Deamidated (NQ)
3660   1105.5756 2209.1366 2209.1226 6.35 0 138 1.2e-013 +1Score > 22 indicates identity
Score > 21 indicates homology		 U R.THQALEGAEDLLTDLLDISR.L
3662 +1 737.7246 2210.1520 2210.1066 20.5 0 67 1.8e-006 +1Score > 22 indicates identity U R.THQALEGAEDLLTDLLDISR.L + Deamidated (NQ)
3676   750.4237 2248.2493 2248.2216 12.3 0 46 0.00011 +1Score > 19 indicates identity U K.EVPLAVHQAVPLPSVGDPLPGR.R + Deamidated (NQ)
3794   602.0956 2404.3533 2404.3227 12.7 1 21 0.029 +1Score > 18 indicates identity U K.EVPLAVHQAVPLPSVGDPLPGRR.L + Deamidated (NQ)
3820   816.7657 2447.2753 2447.2445 12.6 1 61 6.9e-006 +1Score > 22 indicates identity U R.GIAQDRLQDIFLEFNQLDVGR.A + Deamidated (NQ)
4233   848.7004 3390.7725 3390.7130 17.6 0 89 9.2e-009 +1Score > 21 indicates identity U K.DGSHLDVLMNLLLKPGHEGLVEGFVADITER.K + Oxidation (M); Deamidated (NQ)
4234   679.1639 3390.7831 3390.7130 20.7 0 34 0.00089 +1Score > 21 indicates identity
Score > 16 indicates homology		 U K.DGSHLDVLMNLLLKPGHEGLVEGFVADITER.K + Oxidation (M); Deamidated (NQ)
4259   704.7818 3518.8726 3518.8079 18.4 1 7 1 +1Score > 20 indicates identity
Score > 20 indicates homology		 U K.DGSHLDVLMNLLLKPGHEGLVEGFVADITERK.L + Oxidation (M); Deamidated (NQ)
4283   748.2036 3735.9816 3735.9319 13.3 1 33 0.0033 +1Score > 20 indicates identity U R.THQALEGAEDLLTDLLDISRLDQAAVKPDVAVYR.L + Deamidated (NQ)
4284   935.2512 3736.9757 3736.9159 16.0 1 15 0.22 +1Score > 21 indicates identity U R.THQALEGAEDLLTDLLDISRLDQAAVKPDVAVYR.L + 2 Deamidated (NQ)

2 subsets and intersections (2 subset proteins in total)

Score Mass Subset of
Q88CR4_PSEPK 39 49354 2.1
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5116 PE=3 SV=1
Q88NV0_PSEPK 35 0 2.1
description

+3

Accession Score Description
1 TRYP_PIG 212 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q88HN7_PSEPK 173 Chaperone-associated ATPase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3316 PE=4 SV=1

+5

Accession Score Description
1 Q88QY6_PSEPK 50 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0348 PE=4 SV=1

+6

Accession Score Description
1 CH60_PSEPK 47 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

+7

Accession Score Description
1 Q88FW8_PSEPK 42 Voltage-gated chloride channel family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3959 PE=4 SV=1

+8

Accession Score Description
1 Q88H31_PSEPK 37 description

+9

Accession Score Description
1 Q88RB6_PSEPK 36 Fatty acid desaturase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_0217 PE=4 SV=1

+10

Accession Score Description
1 Q88MB8_PSEPK 34 GTP pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=relA PE=3 SV=1
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