MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 36)

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+11

Accession Score Description
1 Q88H50_PSEPK 33 Hydantoin utilization protein A OS=Pseudomonas putida (strain KT2440) GN=hyuA PE=4 SV=1

+12

Accession Score Description
1 Q88M80_PSEPK 32 description

+13

Accession Score Description
1 NICB_PSEPK 32 Nicotinate dehydrogenase subunit B OS=Pseudomonas putida (strain KT2440) GN=nicB PE=1 SV=1

+14

Accession Score Description
1 Q88N36_PSEPK 31 3-oxoadipate enol-lactone hydrolase OS=Pseudomonas putida (strain KT2440) GN=pcaD PE=4 SV=1

+15

Accession Score Description
1 Q88NE1_PSEPK 27 description

+16

Accession Score Description
1 Q88GZ2_PSEPK 27 Monooxygenase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3573 PE=4 SV=1

+17

Accession Score Description
1 Q7CCB3_PSEPK 26 Transcriptional regulator, LysR family OS=Pseudomonas putida (strain KT2440) GN=PP_4494 PE=4 SV=1

-18

Accession Score Description
1 Q88J30_PSEPK 26 Outer membrane protein OprJ OS=Pseudomonas putida (strain KT2440) GN=oprJ PE=3 SV=1
Score Mass Matches Sequences emPAI
18.1 Q88J30_PSEPK 26 50514 4 (1) 3 (1) 0.07
Outer membrane protein OprJ OS=Pseudomonas putida (strain KT2440) GN=oprJ PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2366   390.6954 779.3762 779.3670 11.9 0 10 0.57 +6Score > 20 indicates identity U M.MIMVNR.V + Oxidation (M); Deamidated (NQ)
2367   780.3839 779.3766 779.3670 12.4 0 13 0.28 +3Score > 20 indicates identity U M.MIMVNR.V + Oxidation (M); Deamidated (NQ)
2640   537.3067 1072.5988 1072.6393 -37.7 0 8 1.5 +6Score > 22 indicates identity U R.QLALVNLFR.A
3891   850.0551 2547.1435 2547.1877 -17.4 1 26 0.016 +1Score > 21 indicates identity U R.YLDAQRNSYVNEAAFIEASTQR.Q + 2 Deamidated (NQ)

+19

Accession Score Description
1 Q88KU6_PSEPK 25 Outer membrane ferric siderophore receptor OS=Pseudomonas putida (strain KT2440) GN=PP_2193 PE=3 SV=1

+20

Accession Score Description
1 Q88P58_PSEPK 23 description
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