MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)

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+1

Accession Score Description
1 AMYA3_ASPOR 681 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 407 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 Q2UB64_ASPOR 297 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 291 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UG98_ASPOR 286 Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 282 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 EGLC_ASPOR 268 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+8

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

-9

Accession Score Description
1 Q2U8Y3_ASPOR 252 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1
Score Mass Matches Sequences emPAI
9.1 Q2U8Y3_ASPOR 252 31694 7 (7) 7 (7) 1.01
Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

-7 peptide matches (7 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4018   491.2168 980.4190 980.4134 5.75 0 52 7.2e-005 +1Score > 23 indicates identity U R.SGPCGYNAR.D
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.raw

Score > 27 indicates identity
4095   603.2998 1204.5850 1204.5758 7.69 0 66 8.5e-006 -1Score > 27 indicates identity U R.ICQDQSIVDK.F
-14.3 1 22 0.21 2 LCQSVQKWR   + Deamidated (NQ)
-14.3 1 17 0.66 3 LCQSVQKWR   + Deamidated (NQ)
-37.2 0 12 1.9 4 SSVEVESLLSR  
-22.5 1 11 2.4 5 ICQAIKSQQK   + 2 Deamidated (NQ)
44.1 0 10 3.1 6 GSENGQADALSR   + Deamidated (NQ)
-22.5 1 10 3.1 7 ICQAIKSQQK   + 2 Deamidated (NQ)
-22.5 1 8 4.5 8 ICQAIKSQQK   + 2 Deamidated (NQ)
7.70 0 8 4.8 9 QLAVEMDQVR   + Oxidation (M); Deamidated (NQ)
-47.3 1 8 5.1 10 LCRVGQMLTK  
4167   769.8765 1537.7384 1537.7300 5.48 0 66 7.3e-006 +1Score > 27 indicates identity U K.FLDPSYLPTNDEK.Q
4243   648.6201 1942.8385 1942.8268 6.00 0 74 1.3e-007 +1Score > 24 indicates identity
Score > 17 indicates homology		 U R.NDWFTCNGFEASDRPK.C
4323   870.3980 2608.1722 2608.1421 11.5 0 21 0.02 +1Score > 26 indicates identity
Score > 17 indicates homology		 U K.CQGVDNAELNSCYTSIAGGYTVTK.K + Deamidated (NQ)
4330   909.4463 2725.3171 2725.2793 13.9 1 34 0.00097 +1Score > 27 indicates identity
Score > 17 indicates homology		 U R.ICQDQSIVDKFLDPSYLPTNDEK.Q + Deamidated (NQ)
4331   913.0972 2736.2698 2736.2371 11.9 1 71 1.7e-006 +1Score > 26 indicates identity U K.CQGVDNAELNSCYTSIAGGYTVTKK.V + Deamidated (NQ)

+10

Accession Score Description
1 ABNC_ASPOR 229 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
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