MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)

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+1

Accession Score Description
1 AMYA3_ASPOR 681 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 407 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 Q2UB64_ASPOR 297 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 291 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UG98_ASPOR 286 Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 282 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

-7

Accession Score Description
1 EGLC_ASPOR 268 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1
Score Mass Matches Sequences emPAI
7.1 EGLC_ASPOR 268 46742 9 (9) 9 (9) 0.85
Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3973   429.2143 856.4140 856.4079 7.16 0 42 0.00024 +1Score > 24 indicates identity
Score > 18 indicates homology		 U K.GFNYGATK.S
4019   491.2510 980.4874 980.4815 6.09 0 40 0.0024 +1Score > 26 indicates identity U K.SLFDDSVAK.T
4096   605.3186 1208.6226 1208.6150 6.36 0 40 0.00036 +1Score > 26 indicates identity
Score > 18 indicates homology		 U K.NLVGTSGFTSAR.L
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.raw

Score > 27 indicates identity
4120   637.3111 1272.6076 1272.5986 7.10 0 60 2.4e-005 -1Score > 27 indicates identity U K.SAISQYGDSFAK.L
-1.72 0 18 0.37 2 ASSIYQHNVPR   + 2 Deamidated (NQ)
-13.7 1 8 4.3 3 DVEAWWKSPR  
-42.3 0 1 19 4 FELIHFTNPR  
-41.8 1 1 19 4 MVEAARLSQPR   + Oxidation (M)
1.42 1 1 19 4 RNQSVDTHSTK   + Deamidated (NQ)
-46.9 0 1 19 7 ALSSPNSQVTLR   + Deamidated (NQ)
-22.5 1 0 23 8 FNPKGAYHPSR  
1.28 0 0 23 9 ASILFDFGEMK   + Oxidation (M)
12.2 0 0 23 9 TGHMPFVSQPR   + Oxidation (M); Deamidated (NQ)
4154   731.8240 1461.6334 1461.6260 5.12 0 79 8e-008 +1Score > 24 indicates identity
Score > 20 indicates homology		 U K.SQSDFESEFSTAK.N
4165   753.9169 1505.8192 1505.8090 6.83 0 66 5.1e-006 +1Score > 25 indicates identity U K.LVVGISVGSEDLYR.A
4193   857.9783 1713.9420 1713.9301 6.94 0 41 0.00022 +1Score > 25 indicates identity
Score > 17 indicates homology		 U K.VNAGIGIGPDDLVSFIK.E
4301   801.7573 2402.2501 2402.2329 7.14 1 37 0.00056 +1Score > 26 indicates identity
Score > 17 indicates homology		 U R.ASSEGEKVNAGIGIGPDDLVSFIK.E
4355   1001.5266 3001.5580 3001.5033 18.2 1 26 0.0041 +1Score > 25 indicates identity
Score > 15 indicates homology		 U K.GKEVWITETGWPVSGSTQNLGVASLANAK.T + 2 Deamidated (NQ)

+8

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+9

Accession Score Description
1 Q2U8Y3_ASPOR 252 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+10

Accession Score Description
1 ABNC_ASPOR 229 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
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