MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (35 proteins)


Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 AMYA3_ASPOR 681 55283 33 (25) 17 (15) 3.25 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1
2 LAPA_ASPOR 407 41308 13 (11) 12 (11) 1.33 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1
3 Q2UB64_ASPOR 297 28038 11 (7) 9 (6) 1.19 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1
4 ORYZ_ASPOR 291 42546 16 (9) 9 (5) 0.96 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2
5 Q2UG98_ASPOR 286 49531 10 (8) 10 (8) 0.67 Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1
6 P20D2_ASPOR 282 45784 12 (10) 12 (10) 1.01 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1
7 EGLC_ASPOR 268 46742 9 (9) 9 (9) 0.85 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1
8 TRYP_PIG 261 25078 9 (9) 4 (4) 1.72 Trypsin OS=Sus scrofa PE=1 SV=1
9 Q2U8Y3_ASPOR 252 31694 7 (7) 7 (7) 1.01 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1
10 ABNC_ASPOR 229 34396 15 (9) 8 (5) 1.09 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
11 Q2U2Z5_ASPOR 218 14669 11 (10) 5 (5) 7.07 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
12 GFP_AEQVI 192 26983 9 (8) 7 (6) 1.54 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1
13 Q2U9Z9_ASPOR 191 59229 7 (5) 7 (5) 0.31 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000599 PE=3 SV=1
14 AMYG_ASPOR 187 65929 12 (9) 11 (9) 0.55 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2
15 RNT2_ASPOR 163 31293 12 (8) 9 (6) 1.24 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2
16 Q2ULW8_ASPOR 163 25695 5 (5) 5 (5) 0.84 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1
17 Q2UTD2_ASPOR 149 33809 9 (6) 8 (6) 0.75 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1
18 X5DSL3_ANAMA 82 26705 5 (3) 5 (3) 0.42 MCherry fluorescent protein OS=Anaplasma marginale OX=770 GN=mCherry PE=1 SV=1
19 LECF_ASPOR 52 34690 4 (2) 4 (2) 0.20 Fucose-specific lectin OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=fleA PE=1 SV=1
20 Q2U428_ASPOR 52 52539 4 (3) 4 (3) 0.20 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000517 PE=3 SV=1
21 LAP2_ASPOR 48 53694 2 (2) 2 (2) 0.13 probable leucine aminopeptidase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lap2 PE=1 SV=1
22 Q2UA38_ASPOR 46 17113 1 (1) 1 (1) 0.20 Nucleoside diphosphate kinase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000558 PE=3 SV=1
23 Q2UGH7_ASPOR 45 87338 1 (1) 1 (1) 0.04 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000837 PE=3 SV=1
24 AMPP1_ASPOR 45 73009 1 (1) 1 (1) 0.04 Probable Xaa-Pro aminopeptidase P OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=ampp PE=2 SV=2
25 Q2ULM8_ASPOR 37 33233 2 (1) 2 (1) 0.10 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000346 PE=4 SV=1
26 Q2UUL9_ASPOR 37 35395 3 (2) 3 (2) 0.20 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000264 PE=4 SV=1
27 K1C10_HUMAN 30 59020 2 (2) 2 (2) 0.11 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
28 Q2TZY8_ASPOR 33 29882 1 (1) 1 (1) 0.11 Exonuclease domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000664 PE=4 SV=1
29 Q2UR06_ASPOR 30 19486 1 (1) 1 (1) 0.17 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001025 PE=4 SV=1
30 G6PI_ASPOR 27 61176 1 (1) 1 (1) 0.05 Glucose-6-phosphate isomerase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=pgiA PE=2 SV=1
31 Q2U0Z5_ASPOR 25 65490 1 (1) 1 (1) 0.05 Peptidase S53 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000235 PE=4 SV=1
32 Q2UU21_ASPOR 23 31604 7 (1) 6 (1) 0.11 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000493 PE=4 SV=1
33 Q2U218_ASPOR 23 42811 2 (1) 2 (1) 0.08 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090138000006 PE=4 SV=1
34 Q2TW93_ASPOR 17 19014 1 (1) 1 (1) 0.18 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090010000651 PE=4 SV=1
35 CCR4_ASPOR 17 84350 1 (1) 1 (1) 0.04 Glucose-repressible alcohol dehydrogenase transcriptional effector OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=ccr4 PE=3 SV=1

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