MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 1–10 (out of 35)

Page: 1 2 3 4 Next 

+1

Accession Score Description
1 AMYA3_ASPOR 681 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 407 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 Q2UB64_ASPOR 297 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 291 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UG98_ASPOR 286 Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1

-6

Accession Score Description
1 P20D2_ASPOR 282 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1
Score Mass Matches Sequences emPAI
6.1 P20D2_ASPOR 282 45784 12 (10) 12 (10) 1.01
Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

-12 peptide matches (12 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3887   315.6837 629.3528 629.3497 5.05 0 11 0.68 +1Score > 28 indicates identity
Score > 22 indicates homology		 U R.ADVAVR.L
4002   457.2798 912.5450 912.5392 6.38 0 54 5.4e-005 +1Score > 23 indicates identity U K.LIEAALQR.R
4028   500.7601 999.5056 999.4985 7.15 0 79 2.5e-007 +1Score > 26 indicates identity U R.LAAGTPDEAR.D
4045   521.3279 1040.6412 1040.6342 6.79 1 41 0.00045 +1Score > 20 indicates identity U R.KLIEAALQR.R
4058   540.7951 1079.5756 1079.5685 6.61 0 41 0.00027 +1Score > 26 indicates identity
Score > 18 indicates homology		 U K.GMLGFEIVAK.G + Oxidation (M)
4088   585.3148 1168.6150 1168.6088 5.35 0 22 0.024 +2Score > 26 indicates identity
Score > 18 indicates homology		 U R.VDQLGNIPADK.G
4089   592.8572 1183.6998 1183.6924 6.26 0 51 8.4e-005 +1Score > 22 indicates identity U R.SAISAVLPALSR.V
4144   705.8938 1409.7730 1409.7627 7.35 0 65 5.4e-006 +1Score > 25 indicates identity U R.VDAGVAANVVPATAR.A
4160   495.2700 1482.7882 1482.7790 6.16 1 25 0.061 +1Score > 26 indicates identity
Score > 26 indicates homology		 U R.LAAGTPDEARDIVR.R
4214   893.3962 1784.7778 1784.7489 16.2 0 61 1.2e-005 +1Score > 25 indicates identity
Score > 24 indicates homology		 U R.DATDGNPDVYAEFNTR.S + Deamidated (NQ)
4239   631.6790 1892.0152 1892.0003 7.84 1 43 0.00083 +1Score > 25 indicates identity U R.VDQLGNIPADKGGLPSSPK.Y
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.raw

Score > 26 indicates identity

Score > 20 indicates homology
4353   746.3790 2981.4869 2981.4519 11.7 0 24 0.023 -1Score > 26 indicates identity
Score > 20 indicates homology		 U R.YLYGPGSIHVAHGDNEAITVGDLQEAVR.G + Deamidated (NQ)
40.1 1 7 1.4 2 IATDDNARESPFGEGGALTPNSTTMSVSR   + Deamidated (NQ)
30.4 1 2 3.8 3 AYAAAVMSQTVVKIQTQNPYDEPDAPR   + Oxidation (M); 3 Deamidated (NQ)
30.4 1 2 3.8 3 AYAAAVMSQTVVKIQTQNPYDEPDAPR   + Oxidation (M); 3 Deamidated (NQ)
30.4 1 2 3.8 3 AYAAAVMSQTVVKIQTQNPYDEPDAPR   + Oxidation (M); 3 Deamidated (NQ)
29.2 0 1 4.9 6 LNQMNIISIDDNDIVTASSVPMQSAFR   + 3 Deamidated (NQ)
29.2 0 1 4.9 6 LNQMNIISIDDNDIVTASSVPMQSAFR   + 3 Deamidated (NQ)
29.2 0 1 4.9 6 LNQMNIISIDDNDIVTASSVPMQSAFR   + 3 Deamidated (NQ)

+7

Accession Score Description
1 EGLC_ASPOR 268 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+8

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+9

Accession Score Description
1 Q2U8Y3_ASPOR 252 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+10

Accession Score Description
1 ABNC_ASPOR 229 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
Page: 1 2 3 4 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/