MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)

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+1

Accession Score Description
1 AMYA3_ASPOR 681 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 407 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 Q2UB64_ASPOR 297 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 291 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

-5

Accession Score Description
1 Q2UG98_ASPOR 286 Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1
Score Mass Matches Sequences emPAI
5.1 Q2UG98_ASPOR 286 49531 10 (8) 10 (8) 0.67
Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1

-10 peptide matches (10 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4044   520.8126 1039.6106 1039.6026 7.76 0 46 0.00014 +1Score > 22 indicates identity
Score > 20 indicates homology		 U K.ALAVVSVPER.V
4079   564.8133 1127.6120 1127.6015 9.33 0 47 0.00042 +1Score > 26 indicates identity U K.FLGFEQIFK.N
4082   574.7941 1147.5736 1147.5656 7.05 0 42 0.00011 +1Score > 27 indicates identity
Score > 15 indicates homology		 U K.NALTGLNMGGGK.G + Oxidation (M)
4099   608.3159 1214.6172 1214.6084 7.27 0 40 0.0027 +1Score > 27 indicates identity U R.EVGFLFGQYR.K
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.raw

Score > 26 indicates identity
4101   612.3289 1222.6432 1222.6346 7.06 0 76 6.2e-007 -1Score > 26 indicates identity U R.VQFNSALGPYK.G
-22.7 1 12 1.5 2 VKFYQPSVQK  
4.32 1 12 1.5 3 QVSEMKNFLK  
49.3 0 3 11 4 TFTGGLNQLDR   + 2 Deamidated (NQ)
43.3 0 3 13 5 QVMDFLNIDK   + Deamidated (NQ)
-14.1 1 2 13 6 NLCLHPSVKR  
43.3 0 1 20 7 NLDEFVEVMK  
34.1 0 0 22 8 VQVMAPSNFSK   + Oxidation (M)
7.06 1 0 22 9 RAPFDLFETK  
4114   418.9146 1253.7220 1253.7132 7.00 0 12 0.11 +1Score > 22 indicates identity
Score > 14 indicates homology		 U R.FHPSVNLSILK.F
4161   745.4136 1488.8126 1488.8035 6.12 0 97 1.7e-009 +1Score > 25 indicates identity
Score > 22 indicates homology		 U K.VIELGGSVVSLSDSK.G
4169   774.9270 1547.8394 1547.8307 5.63 0 11 1 +1Score > 25 indicates identity
Score > 24 indicates homology		 U R.VAISGSGNVAQYAALK.V
4202   579.9521 1736.8345 1736.8231 6.57 0 74 6.3e-007 +1Score > 27 indicates identity
Score > 24 indicates homology		 U R.VVWEDDNHQVQVNR.G
4209   584.2997 1749.8773 1749.8646 7.24 0 21 0.039 +1Score > 27 indicates identity
Score > 20 indicates homology		 U K.HIGADTDVPAGDIGVTGR.E

+6

Accession Score Description
1 P20D2_ASPOR 282 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 EGLC_ASPOR 268 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+8

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+9

Accession Score Description
1 Q2U8Y3_ASPOR 252 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+10

Accession Score Description
1 ABNC_ASPOR 229 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
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