MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Ofer16 |
| MS data file | : | Ofer16.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 7 May 2026 at 18:12:20 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,508 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMYA3_ASPOR | 681 | Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LAPA_ASPOR | 407 | Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UB64_ASPOR | 297 | SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ORYZ_ASPOR | 291 | Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 4.1 | ORYZ_ASPOR |
291 | 42546 | 16 (9) | 9 (5) | 0.96 | |
| Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2 | |||||||
16 peptide matches (16 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 3894 |
326.6889 | 651.3632 | 651.3592 | 6.27 | 0 | 28 | 0.0036 | 1Score > 24 indicates identity Score > 16 indicates homology |
U | K.TYGIAK.K | |
| 3895 |
652.3708 | 651.3635 | 651.3592 | 6.70 | 0 | 34 | 0.0027 | 1Score > 24 indicates identity Score > 21 indicates homology |
U | K.TYGIAK.K | |
| 3922 |
717.4537 | 716.4464 | 716.4432 | 4.47 | 0 | 6 | 1.2 | 2Score > 23 indicates identity Score > 20 indicates homology |
U | K.ASILSVK.V | |
| 3923 |
359.2308 | 716.4470 | 716.4432 | 5.34 | 0 | 17 | 0.24 | 1Score > 23 indicates identity |
U | K.ASILSVK.V | |
| 3942 |
401.7398 | 801.4650 | 801.4596 | 6.77 | 1 | 15 | 1 | 3Score > 27 indicates identity |
U | K.DVVKDVK.G | |
| 3965 |
423.2791 | 844.5436 | 844.5382 | 6.48 | 1 | 42 | 0.00029 | 1Score > 19 indicates identity |
U | K.KASILSVK.V | |
| 3971 |
429.2135 | 856.4124 | 856.4079 | 5.30 | 0 | 48 | 3e-005 | 1Score > 24 indicates identity Score > 16 indicates homology |
U | R.ASFSNFGK.V | |
| 3972 |
857.4203 | 856.4130 | 856.4079 | 5.97 | 0 | 36 | 0.00043 | 1Score > 24 indicates identity Score > 15 indicates homology |
U | R.ASFSNFGK.V | |
| 4094 |
596.2901 | 1190.5656 | 1190.5567 | 7.49 | 0 | 11 | 0.87 | 1Score > 27 indicates identity Score > 23 indicates homology |
U | K.GSPNLLAYNGNA.- + Deamidated (NQ) | |
| 4106 |
620.3318 | 1238.6490 | 1238.6408 | 6.69 | 0 | 60 | 1e-005 | 1Score > 26 indicates identity Score > 23 indicates homology |
U | K.SAPWGLGSISHK.G | |
| 4107 |
413.8904 | 1238.6494 | 1238.6408 | 6.95 | 0 | 41 | 0.00061 | 1Score > 26 indicates identity Score > 22 indicates homology |
U | K.SAPWGLGSISHK.G | |
| 4119 |
634.8234 | 1267.6322 | 1267.6230 | 7.26 | 0 | 76 | 1.2e-007 | 1Score > 26 indicates identity Score > 19 indicates homology |
U | K.AAINMSLGGGYSK.A | |
| 4125 |
642.8211 | 1283.6276 | 1283.6180 | 7.54 | 0 | 80 | 1.2e-007 | 1Score > 26 indicates identity Score > 23 indicates homology |
U | K.AAINMSLGGGYSK.A + Oxidation (M) | |
| 4126 |
643.3325 | 1284.6504 | 1284.6020 | 37.7 | 0 | 12 | 0.99 | 1Score > 27 indicates identity Score > 25 indicates homology |
U | K.AAINMSLGGGYSK.A + Oxidation (M); Deamidated (NQ) | |
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.rawScore > 27 indicates identity |
|||||||||||
| 4313 |
502.0548 | 2505.2376 | 2505.1997 | 15.1 | 0 | 24 | 0.11 | 1Score > 27 indicates identity |
U | K.AYNAAGGQHVDSIGHGTHVSGTIAGK.T + Deamidated (NQ) | |
| 15.1 | 0 | 22 | 0.17 | 2 | K.AYNAAGGQHVDSIGHGTHVSGTIAGK.T + Deamidated (NQ) | ||||||
| 4314 |
627.3172 | 2505.2397 | 2505.1997 | 16.0 | 0 | 18 | 0.42 | 1Score > 27 indicates identity |
U | K.AYNAAGGQHVDSIGHGTHVSGTIAGK.T + Deamidated (NQ) | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UG98_ASPOR | 286 | Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | P20D2_ASPOR | 282 | Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EGLC_ASPOR | 268 | Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 261 | Trypsin OS=Sus scrofa PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U8Y3_ASPOR | 252 | Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ABNC_ASPOR | 229 | Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1 |
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