MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)

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+1

Accession Score Description
1 AMYA3_ASPOR 681 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 407 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

-3

Accession Score Description
1 Q2UB64_ASPOR 297 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1
Score Mass Matches Sequences emPAI
3.1 Q2UB64_ASPOR 297 28038 11 (7) 9 (6) 1.19
SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

-11 peptide matches (11 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3930   374.1873 746.3600 746.3559 5.62 0 15 1.1 +5Score > 28 indicates identity U R.SLENER.V
3938   394.7318 787.4490 787.4440 6.47 0 14 0.25 +1Score > 27 indicates identity
Score > 21 indicates homology		 U K.LQTALDK.G
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.raw

Score > 28 indicates identity

Score > 28 indicates homology
4053   538.2778 1074.5410 1074.5346 6.02 0 68 4.9e-006 -1Score > 28 indicates identity
Score > 28 indicates homology		 U R.AGGDWDTLLK.K
-7.52 1 11 2.7 2 QAMRAQLQK   + 2 Deamidated (NQ)
20.2 0 8 4.8 3 ATGAGEIDDVK  
-41.4 0 5 9.8 4 GAMLALGTSVR  
-11.3 0 5 11 5 QGLAWMIEK  
-13.6 0 5 11 6 AVSADDVTGLK  
-0.67 1 4 12 7 QATRTNVER   + Deamidated (NQ)
-0.67 1 4 14 8 ASQTDLRQR   + Deamidated (NQ)
-38.3 1 3 15 9 GAFPGTGVKGGK  
20.2 0 3 15 10 QVTENDELK  
4069   555.2852 1108.5558 1108.5513 4.13 0 65 7.6e-006 +1Score > 26 indicates identity U K.TGASYIDLNR.A
4104   619.3358 1236.6570 1236.6462 8.75 1 75 6.2e-007 +1Score > 26 indicates identity U K.KTGASYIDLNR.A
4138   690.4084 1378.8022 1378.7933 6.52 0 85 2.7e-008 +1Score > 22 indicates identity U R.NAGGVPVLVTPLSR.R
4177   805.3754 1608.7362 1608.7281 5.07 0 26 0.0074 +1Score > 26 indicates identity
Score > 17 indicates homology		 U K.GQNYGHNGATTVSFR.A + Deamidated (NQ)
4178   537.2545 1608.7417 1608.7281 8.44 0 62 3e-006 +1Score > 27 indicates identity
Score > 19 indicates homology		 U K.GQNYGHNGATTVSFR.A + Deamidated (NQ)
4192   856.4339 1710.8532 1710.8253 16.3 1 5 1.3 +3Score > 27 indicates identity
Score > 18 indicates homology		 U K.LQTALDKGQYYWPQ.- + Deamidated (NQ)
4267   723.0383 2166.0931 2166.0667 12.2 0 6 0.39 +1Score > 27 indicates identity
Score > 14 indicates homology		 U R.ASTVYLNSIGPVSAHMYDLK.T + Deamidated (NQ)
4270   728.3712 2182.0918 2182.0616 13.8 0 48 3.1e-005 +1Score > 27 indicates identity
Score > 16 indicates homology		 U R.ASTVYLNSIGPVSAHMYDLK.T + Oxidation (M); Deamidated (NQ)

+4

Accession Score Description
1 ORYZ_ASPOR 291 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UG98_ASPOR 286 Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 282 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 EGLC_ASPOR 268 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+8

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+9

Accession Score Description
1 Q2U8Y3_ASPOR 252 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+10

Accession Score Description
1 ABNC_ASPOR 229 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
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