MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 35)

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+21

Accession Score Description
1 LAP2_ASPOR 48 probable leucine aminopeptidase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lap2 PE=1 SV=1

+22

Accession Score Description
1 Q2UA38_ASPOR 46 Nucleoside diphosphate kinase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000558 PE=3 SV=1

+23

Accession Score Description
1 Q2UGH7_ASPOR 45 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000837 PE=3 SV=1

+24

Accession Score Description
1 AMPP1_ASPOR 45 Probable Xaa-Pro aminopeptidase P OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=ampp PE=2 SV=2

+25

Accession Score Description
1 Q2ULM8_ASPOR 37 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000346 PE=4 SV=1

+26

Accession Score Description
1 Q2UUL9_ASPOR 37 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000264 PE=4 SV=1

-27

Accession Score Description
1 K1C10_HUMAN 30 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
Score Mass Matches Sequences emPAI
27.1 K1C10_HUMAN 30 59020 2 (2) 2 (2) 0.11
Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3945   404.2066 806.3986 806.3923 7.92 0 34 0.01 +1Score > 27 indicates identity U R.LAADDFR.L
4162   498.5865 1492.7377 1492.7270 7.17 1 18 0.048 +1Score > 27 indicates identity
Score > 17 indicates homology		 U R.SQYEQLAEQNRK.D

1 subset or intersection (10 subset proteins in total)

Score Mass Subset of
K1C15_SHEEP 34 48740 27.1
Keratin, type I cytoskeletal 15 OS=Ovis aries GN=KRT15 PE=2 SV=1
9 samesets of K1C15_SHEEP
K1H1_HUMAN 34 48633
Keratin, type I cuticular Ha1 OS=Homo sapiens GN=KRT31 PE=2 SV=3
K1H2_HUMAN 34 51793
Keratin, type I cuticular Ha2 OS=Homo sapiens GN=KRT32 PE=1 SV=3
K1M1_SHEEP 34 48070
Keratin, type I microfibrillar 48 kDa, component 8C-1 OS=Ovis aries PE=1 SV=2
Q2UHT3_ASPOR 34 46483
Protein phosphatase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000321 PE=3 SV=1
KT33B_HUMAN 34 47325
Keratin, type I cuticular Ha3-II OS=Homo sapiens GN=KRT33B PE=2 SV=3
KRT35_HUMAN 34 51640
Keratin, type I cuticular Ha5 OS=Homo sapiens GN=KRT35 PE=2 SV=5
KRT36_HUMAN 34 53354
Keratin, type I cuticular Ha6 OS=Homo sapiens GN=KRT36 PE=1 SV=1
KRT37_HUMAN 34 51084
Keratin, type I cuticular Ha7 OS=Homo sapiens GN=KRT37 PE=2 SV=3
KRT38_HUMAN 34 52044
Keratin, type I cuticular Ha8 OS=Homo sapiens GN=KRT38 PE=2 SV=3

+28

Accession Score Description
1 Q2TZY8_ASPOR 33 Exonuclease domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000664 PE=4 SV=1

+29

Accession Score Description
1 Q2UR06_ASPOR 30 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001025 PE=4 SV=1

+30

Accession Score Description
1 G6PI_ASPOR 27 Glucose-6-phosphate isomerase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=pgiA PE=2 SV=1
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