MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Ofer16 |
| MS data file | : | Ofer16.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 7 May 2026 at 18:12:20 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,508 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMYA3_ASPOR | 681 | Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LAPA_ASPOR | 407 | Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 2.1 | LAPA_ASPOR |
407 | 41308 | 13 (11) | 12 (11) | 1.33 | |
| Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 | |||||||
13 peptide matches (13 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 3951 |
409.2394 | 816.4642 | 816.4593 | 6.09 | 0 | 48 | 0.00044 | 1Score > 27 indicates identity |
U | R.VLLTDEK.V | |
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.rawScore > 26 indicates identity Score > 24 indicates homology |
|||||||||||
| 3986 |
438.7474 | 875.4802 | 875.4753 | 5.67 | 0 | 39 | 0.0019 | 1Score > 26 indicates identity Score > 24 indicates homology |
U | K.GVTVEPFK.H | |
| 47.3 | 0 | 9 | 1.7 | 2 | FPQLEDK | ||||||
| -11.0 | 1 | 8 | 2 | 3 | RVTIMQK + Deamidated (NQ) | ||||||
| -7.14 | 1 | 8 | 2.1 | 4 | RDLTFPK | ||||||
| -19.9 | 1 | 8 | 2.3 | 5 | RFAAINGK | ||||||
| 43.4 | 1 | 8 | 2.5 | 6 | KQPMEVK + Oxidation (M); Deamidated (NQ) | ||||||
| 1.89 | 1 | 7 | 2.6 | 7 | KELNMIK + Deamidated (NQ) | ||||||
| -20.0 | 1 | 7 | 2.6 | 8 | RTFPSIR | ||||||
| -48.7 | 1 | 7 | 2.9 | 9 | RDFVVLK | ||||||
| 1.87 | 1 | 6 | 3.3 | 10 | KLDEMLK | ||||||
| 4009 |
467.7384 | 933.4622 | 933.4556 | 7.14 | 0 | 72 | 1.6e-006 | 1Score > 27 indicates identity |
U | K.SSEWLQGK.I | |
| 4074 |
558.8199 | 1115.6252 | 1115.6186 | 5.95 | 0 | 77 | 4.8e-007 | 1Score > 26 indicates identity |
U | K.IQEIISASGAK.G | |
| 4098 |
607.8325 | 1213.6504 | 1213.6455 | 4.07 | 0 | 36 | 0.0057 | 1Score > 26 indicates identity |
U | K.HSFPQSSLIAK.I | |
| 4100 |
608.3366 | 1214.6586 | 1214.6295 | 24.0 | 0 | 2 | 17 | 6Score > 27 indicates identity |
U | K.HSFPQSSLIAK.I + Deamidated (NQ) | |
| 4145 |
709.8234 | 1417.6322 | 1417.6218 | 7.41 | 0 | 72 | 6e-007 | 1Score > 25 indicates identity Score > 22 indicates homology |
U | K.AMLQQDMTGYTK.G + 2 Oxidation (M) | |
| 4188 |
840.9343 | 1679.8540 | 1679.8440 | 5.96 | 0 | 70 | 4.3e-007 | 1Score > 27 indicates identity Score > 19 indicates homology |
U | K.VIVDAYCTIPTVDSK.C | |
| 4230 |
622.6431 | 1864.9075 | 1864.8744 | 17.7 | 0 | 57 | 6.1e-005 | 1Score > 27 indicates identity |
U | K.NFETVLQPFSEFHNR.Y + Deamidated (NQ) | |
| 4236 |
945.4725 | 1888.9304 | 1888.9167 | 7.29 | 0 | 84 | 1.2e-007 | 1Score > 27 indicates identity |
U | R.APGADDDGSGVVTILEAFR.V | |
| 4258 |
519.0390 | 2072.1269 | 2072.0942 | 15.8 | 1 | 12 | 0.13 | 1Score > 24 indicates identity Score > 16 indicates homology |
U | K.GVTVEPFKHSFPQSSLIAK.I + Deamidated (NQ) | |
| 4261 |
704.0284 | 2109.0634 | 2109.0338 | 14.0 | 0 | 39 | 0.00042 | 1Score > 27 indicates identity Score > 18 indicates homology |
U | K.TIVLGAHQDSINLDSPSEGR.A + Deamidated (NQ) | |
| 4309 |
830.4242 | 2488.2508 | 2488.2180 | 13.2 | 0 | 53 | 3.3e-005 | 1Score > 27 indicates identity Score > 21 indicates homology |
U | K.GTTDAGKPESIGIITDNVDENLTK.F + Deamidated (NQ) | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UB64_ASPOR | 297 | SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ORYZ_ASPOR | 291 | Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UG98_ASPOR | 286 | Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | P20D2_ASPOR | 282 | Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EGLC_ASPOR | 268 | Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 261 | Trypsin OS=Sus scrofa PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U8Y3_ASPOR | 252 | Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ABNC_ASPOR | 229 | Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1 |
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