MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 35)

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+11

Accession Score Description
1 Q2U2Z5_ASPOR 218 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1

+12

Accession Score Description
1 GFP_AEQVI 192 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1

+13

Accession Score Description
1 Q2U9Z9_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000599 PE=3 SV=1

+14

Accession Score Description
1 AMYG_ASPOR 187 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

-15

Accession Score Description
1 RNT2_ASPOR 163 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2
Score Mass Matches Sequences emPAI
15.1 RNT2_ASPOR 163 31293 12 (8) 9 (6) 1.24
Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

-12 peptide matches (12 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3908   682.3449 681.3376 681.3333 6.28 0 18 0.1 +1Score > 24 indicates identity
Score > 21 indicates homology		 U K.GLDSYK.A
3909   341.6762 681.3378 681.3333 6.61 0 18 0.24 +1Score > 24 indicates identity U K.GLDSYK.A
3924   722.4128 721.4055 721.4010 6.21 0 32 0.0099 +1Score > 24 indicates identity U K.TVDLFK.G
3925   361.7103 721.4060 721.4010 6.93 0 43 0.00068 +1Score > 24 indicates identity U K.TVDLFK.G
3983   437.2402 872.4658 872.4603 6.31 0 49 0.00021 +1Score > 28 indicates identity
Score > 25 indicates homology		 U K.AGIVPDSSK.T
4013   476.2458 950.4770 950.4709 6.47 0 39 0.0033 +1Score > 27 indicates identity U K.EVGDYLQK.T
4022   492.7596 983.5046 983.4997 4.99 0 48 9.3e-005 +1Score > 25 indicates identity
Score > 20 indicates homology		 U R.TELLSYMK.K
4027   500.7577 999.5008 999.4947 6.19 0 43 0.0011 +1Score > 26 indicates identity U R.TELLSYMK.K + Oxidation (M)
4078   376.8730 1127.5972 1127.5896 6.70 1 2 1 +1Score > 26 indicates identity
Score > 14 indicates homology		 U R.TELLSYMKK.Y + Oxidation (M)
4186   557.2785 1668.8137 1668.7995 8.49 1 29 0.0041 +1Score > 27 indicates identity
Score > 17 indicates homology		 U K.DYSPQKEVGDYLQK.T
4187   840.3840 1678.7534 1678.7443 5.44 0 16 0.57 +1Score > 26 indicates identity U K.HGTCINTIEPSCYK.D
4320   856.7536 2567.2390 2567.2061 12.8 0 42 0.00048 +1Score > 27 indicates identity
Score > 21 indicates homology		 U K.GNAITGEYQPIDTLTSPGCSTSGIK.Y + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q2UDP7_ASPOR 36 201276 15.1
AAA domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090012000087 PE=4 SV=1

+16

Accession Score Description
1 Q2ULW8_ASPOR 163 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1

+17

Accession Score Description
1 Q2UTD2_ASPOR 149 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1

+18

Accession Score Description
1 X5DSL3_ANAMA 82 MCherry fluorescent protein OS=Anaplasma marginale OX=770 GN=mCherry PE=1 SV=1

+19

Accession Score Description
1 LECF_ASPOR 52 Fucose-specific lectin OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=fleA PE=1 SV=1

+20

Accession Score Description
1 Q2U428_ASPOR 52 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000517 PE=3 SV=1
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