MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 35)

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+11

Accession Score Description
1 Q2U2Z5_ASPOR 218 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1

+12

Accession Score Description
1 GFP_AEQVI 192 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1

+13

Accession Score Description
1 Q2U9Z9_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000599 PE=3 SV=1

-14

Accession Score Description
1 AMYG_ASPOR 187 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2
Score Mass Matches Sequences emPAI
14.1 AMYG_ASPOR 187 65929 12 (9) 11 (9) 0.55
Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

-12 peptide matches (12 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3955   412.2221 822.4296 822.4236 7.37 0 36 0.0045 +1Score > 25 indicates identity
Score > 25 indicates homology		 U K.VVTDSFR.S
3975   432.2567 862.4988 862.4913 8.80 0 42 0.00073 +1Score > 23 indicates identity U K.TLVDLFR.G
4017   490.7651 979.5156 979.5087 7.13 0 31 0.0092 +1Score > 25 indicates identity
Score > 23 indicates homology		 U R.SIYAINSGR.A
4019   491.2510 980.4874 980.4927 -5.33 0 8 3.6 +5Score > 26 indicates identity U R.SIYAINSGR.A + Deamidated (NQ)
4029   504.7745 1007.5344 1007.5287 5.66 0 19 0.14 +1Score > 26 indicates identity
Score > 23 indicates homology		 U R.ALVEGSSFAK.T
4033   507.7737 1013.5328 1013.5254 7.36 0 45 0.00072 +1Score > 26 indicates identity U R.AENQAVAVGR.Y
4048   524.2684 1046.5222 1046.5145 7.39 0 58 4.8e-005 +1Score > 27 indicates identity U K.SAVQSDVWR.-
4050   534.7861 1067.5576 1067.5499 7.28 0 41 0.0003 +1Score > 25 indicates identity
Score > 18 indicates homology		 U K.ATTVYGESIK.I
4070   556.2805 1110.5464 1110.5380 7.64 0 40 0.0021 +1Score > 26 indicates identity U K.YTVPSTCGVK.S
4105   620.3281 1238.6416 1238.6329 7.05 1 4 0.78 +1Score > 26 indicates identity
Score > 15 indicates homology		 U R.KYTVPSTCGVK.S
4176   787.3702 1572.7258 1572.7168 5.73 0 55 9.4e-006 +1Score > 26 indicates identity
Score > 18 indicates homology		 U R.VQNGAVTWESDPNR.K + Deamidated (NQ)
4190   567.9482 1700.8228 1700.8118 6.45 1 26 0.044 +1Score > 27 indicates identity
Score > 25 indicates homology		 U R.VQNGAVTWESDPNRK.Y + Deamidated (NQ)

+15

Accession Score Description
1 RNT2_ASPOR 163 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

+16

Accession Score Description
1 Q2ULW8_ASPOR 163 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1

+17

Accession Score Description
1 Q2UTD2_ASPOR 149 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1

+18

Accession Score Description
1 X5DSL3_ANAMA 82 MCherry fluorescent protein OS=Anaplasma marginale OX=770 GN=mCherry PE=1 SV=1

+19

Accession Score Description
1 LECF_ASPOR 52 Fucose-specific lectin OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=fleA PE=1 SV=1

+20

Accession Score Description
1 Q2U428_ASPOR 52 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000517 PE=3 SV=1
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