MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 35)


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+11

Accession Score Description
1 Q2U2Z5_ASPOR 218 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1

+12

Accession Score Description
1 GFP_AEQVI 192 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1

-13

Accession Score Description
1 Q2U9Z9_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000599 PE=3 SV=1
Score Mass Matches Sequences emPAI
13.1 Q2U9Z9_ASPOR 191 59229 7 (5) 7 (5) 0.31
Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000599 PE=3 SV=1

-7 peptide matches (7 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4085   583.7812 1165.5478 1165.5397 6.95 0 24 0.11 +1Score > 26 indicates identity U R.SGGTIGPMTSSR.I + Oxidation (M)
4090   593.2858 1184.5570 1184.5496 6.32 0 56 6.4e-006 +1Score > 26 indicates identity
Score > 17 indicates homology
U R.SNFMSSVIER.I + Oxidation (M)
4100   608.3366 1214.6586 1214.6507 6.56 0 81 2.4e-007 +1Score > 27 indicates identity U R.ATTGSLDPGLGVK.L
4129   654.8442 1307.6738 1307.6656 6.29 0 24 0.093 +1Score > 27 indicates identity
Score > 27 indicates homology
U R.CGSTLQVFQIR.N
4183   551.9692 1652.8858 1652.8556 18.3 0 30 0.018 +1Score > 25 indicates identity U R.AIDVGIPQLSMHSIR.A + Oxidation (M); Deamidated (NQ)
4205   872.4057 1742.7968 1742.7855 6.50 0 71 2.1e-006 +1Score > 26 indicates identity U K.MVGMFDDEEIGSLLR.Q + 2 Oxidation (M)
4351   979.1324 2934.3754 2934.3474 9.53 1 43 0.00011 +1Score > 26 indicates identity
Score > 16 indicates homology
U R.IDDKLCCYAAQEALLASSDSTSTSSIK.M + Deamidated (NQ)

+14

Accession Score Description
1 AMYG_ASPOR 187 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

+15

Accession Score Description
1 RNT2_ASPOR 163 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

+16

Accession Score Description
1 Q2ULW8_ASPOR 163 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1

+17

Accession Score Description
1 Q2UTD2_ASPOR 149 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1

+18

Accession Score Description
1 X5DSL3_ANAMA 82 MCherry fluorescent protein OS=Anaplasma marginale OX=770 GN=mCherry PE=1 SV=1

+19

Accession Score Description
1 LECF_ASPOR 52 Fucose-specific lectin OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=fleA PE=1 SV=1

+20

Accession Score Description
1 Q2U428_ASPOR 52 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000517 PE=3 SV=1
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