MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)


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+1

Accession Score Description
1 AMYA3_ASPOR 681 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 407 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 Q2UB64_ASPOR 297 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 291 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UG98_ASPOR 286 Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 282 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 EGLC_ASPOR 268 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+8

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+9

Accession Score Description
1 Q2U8Y3_ASPOR 252 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

-10

Accession Score Description
1 ABNC_ASPOR 229 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
Score Mass Matches Sequences emPAI
10.1 ABNC_ASPOR 229 34396 15 (9) 8 (5) 1.09
Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

-15 peptide matches (15 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3899   331.1780 660.3414 660.3377 5.64 0 30 0.028 +1Score > 27 indicates identity U R.INVCR.S
3907   340.6682 679.3218 679.3177 6.08 0 22 0.1 +1Score > 26 indicates identity
Score > 24 indicates homology
U R.GDFVDK.N
4056   360.5275 1078.5607 1078.5560 4.36 0 30 0.0026 +1Score > 26 indicates identity
Score > 17 indicates homology
U K.NLWAPDVHK.G
4057   540.2883 1078.5620 1078.5560 5.64 0 34 0.0094 +1Score > 26 indicates identity U K.NLWAPDVHK.G
4092   1186.5979 1185.5906 1185.5819 7.38 0 10 2.3 +3Score > 27 indicates identity
Score > 26 indicates homology
U R.LNWVDGWPTV.-
4093   593.8040 1185.5934 1185.5819 9.76 0 4 1.1 +2Score > 27 indicates identity
Score > 17 indicates homology
U R.LNWVDGWPTV.-
4170   517.9272 1550.7598 1550.7478 7.75 1 1 1 +1Score > 27 indicates identity
Score > 13 indicates homology
U K.NLWAPDVHKGDDGK.Y
4198   577.2726 1728.7960 1728.7856 6.00 0 39 0.0029 +1Score > 26 indicates identity U R.NGDLSVSSYQFGWNR.L
4199   865.8967 1729.7788 1729.7696 5.34 0 66 4.9e-006 +1Score > 25 indicates identity U R.NGDLSVSSYQFGWNR.L + Deamidated (NQ)
4200   577.6010 1729.7812 1729.7696 6.69 0 54 7.5e-005 +1Score > 26 indicates identity
Score > 25 indicates homology
U R.NGDLSVSSYQFGWNR.L + Deamidated (NQ)
4201   865.9057 1729.7968 1729.7696 15.7 0 70 1.7e-006 +1Score > 26 indicates identity
Score > 25 indicates homology
U R.NGDLSVSSYQFGWNR.L + Deamidated (NQ)
4218   902.8967 1803.7788 1803.7700 4.91 0 65 5e-006 +1Score > 24 indicates identity U K.ANGYDTSFPAQGEEYR.I
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.raw

Score > 24 indicates identity

Score > 23 indicates homology

4219   903.8903 1805.7660 1805.7380 15.5 0 11 1 -1Score > 24 indicates identity
Score > 23 indicates homology
U K.ANGYDTSFPAQGEEYR.I + 2 Deamidated (NQ)
No other peptide matches in query
4220   602.9299 1805.7679 1805.7380 16.5 0 16 0.075 +1Score > 24 indicates identity
Score > 17 indicates homology
U K.ANGYDTSFPAQGEEYR.I + 2 Deamidated (NQ)
4306   817.0391 2448.0955 2448.0652 12.4 1 27 0.0033 +1Score > 25 indicates identity
Score > 15 indicates homology
U K.ANGYDTSFPAQGEEYRINVCR.S + 2 Deamidated (NQ)

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