MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Ofer16 |
| MS data file | : | Ofer16.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 7 May 2026 at 18:12:20 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,508 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMYA3_ASPOR | 681 | Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 1.1 | AMYA3_ASPOR |
681 | 55283 | 33 (25) | 17 (15) | 3.25 | |
| Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1 | |||||||
33 peptide matches (32 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 3868 |
575.3431 | 574.3358 | 574.3326 | 5.57 | 0 | 36 | 0.0096 | 1Score > 28 indicates identity |
U | R.IDTVK.H | |
| 3916 |
348.1917 | 694.3688 | 694.3650 | 5.60 | 0 | 37 | 0.0023 | 1Score > 23 indicates identity |
U | R.NYAISK.D | |
| 3917 |
695.3767 | 694.3694 | 694.3650 | 6.43 | 0 | 19 | 0.14 | 1Score > 23 indicates identity |
U | R.NYAISK.D | |
| 3953 |
410.7242 | 819.4338 | 819.4279 | 7.26 | 0 | 14 | 0.18 | 1Score > 27 indicates identity Score > 20 indicates homology |
U | K.NWPIYK.D | |
| 3967 |
849.4766 | 848.4693 | 848.4644 | 5.86 | 0 | 17 | 0.026 | 1Score > 25 indicates identity Score > 14 indicates homology |
U | R.VLYPTEK.L | |
| 3968 |
425.2426 | 848.4706 | 848.4644 | 7.42 | 0 | 36 | 0.0028 | 1Score > 25 indicates identity Score > 23 indicates homology |
U | R.VLYPTEK.L | |
| 4005 |
1 |
464.7857 | 927.5568 | 927.5501 | 7.25 | 0 | 82 | 9.7e-008 | 1Score > 24 indicates identity |
U | K.LIASANAIR.N |
| 4006 |
930.4614 | 929.4541 | 929.4495 | 5.01 | 0 | 52 | 0.00016 | 1Score > 26 indicates identity |
U | K.DTGFVTYK.N | |
| 4007 |
465.7346 | 929.4546 | 929.4495 | 5.58 | 0 | 10 | 0.45 | 1Score > 26 indicates identity Score > 19 indicates homology |
U | K.DTGFVTYK.N | |
| 4020 |
328.5158 | 982.5256 | 982.5196 | 6.12 | 0 | 53 | 7.7e-005 | 1Score > 24 indicates identity |
U | K.ALSSALHER.G | |
| 4021 |
492.2709 | 982.5272 | 982.5196 | 7.82 | 0 | 76 | 4.4e-007 | 1Score > 24 indicates identity |
U | K.ALSSALHER.G | |
| 4038 |
1026.4741 | 1025.4668 | 1025.4607 | 5.99 | 0 | 17 | 0.32 | 1Score > 24 indicates identity |
U | K.DFWPGYNK.A | |
| 4039 |
513.7410 | 1025.4674 | 1025.4607 | 6.60 | 0 | 20 | 0.052 | 1Score > 25 indicates identity Score > 20 indicates homology |
U | K.DFWPGYNK.A | |
| 4130 |
657.3445 | 1312.6744 | 1312.6663 | 6.21 | 0 | 67 | 4.8e-006 | 1Score > 26 indicates identity |
U | R.FASYTNDIALAK.N | |
| 4131 |
657.8364 | 1313.6582 | 1313.6503 | 6.05 | 0 | 95 | 7.7e-009 | 1Score > 27 indicates identity |
U | R.FASYTNDIALAK.N + Deamidated (NQ) | |
| 4134 |
671.8590 | 1341.7034 | 1341.6929 | 7.89 | 0 | 62 | 1.5e-005 | 1Score > 27 indicates identity |
U | R.SQSIYFLLTDR.F | |
| 4149 |
716.8906 | 1431.7666 | 1431.7569 | 6.79 | 0 | 69 | 3.1e-006 | 1Score > 26 indicates identity Score > 26 indicates homology |
U | K.GTDGSQIVTILSNK.G | |
| 4150 |
478.2631 | 1431.7675 | 1431.7569 | 7.37 | 0 | 42 | 0.00015 | 1Score > 26 indicates identity Score > 16 indicates homology |
U | K.GTDGSQIVTILSNK.G | |
| 4156 |
735.8142 | 1469.6138 | 1469.6053 | 5.84 | 0 | 24 | 0.015 | 1Score > 22 indicates identity Score > 18 indicates homology |
U | R.TDGSTTATCNTADR.K | |
| 4166 |
506.9244 | 1517.7514 | 1517.7415 | 6.47 | 1 | 18 | 0.07 | 1Score > 27 indicates identity Score > 19 indicates homology |
U | K.HVQKDFWPGYNK.A | |
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.rawScore > 25 indicates identity |
|||||||||||
| 4172 |
780.9376 | 1559.8606 | 1559.8519 | 5.62 | 1 | 37 | 0.0031 | 1Score > 25 indicates identity |
U | R.KGTDGSQIVTILSNK.G | |
| 42.5 | 0 | 10 | 1.7 | 2 | QVFLAIEQAEGAGAR + Deamidated (NQ) | ||||||
| 46.7 | 1 | 1 | 13 | 3 | LGGLQQRWCLNSK + Deamidated (NQ) | ||||||
| 46.7 | 1 | 1 | 13 | 3 | LGGLQQRWCLNSK + Deamidated (NQ) | ||||||
| -6.32 | 1 | 0 | 16 | 5 | IMILRNQTGGTIVK + Oxidation (M); Deamidated (NQ) | ||||||
| 4173 |
520.9609 | 1559.8609 | 1559.8519 | 5.76 | 1 | 38 | 0.0017 | 1Score > 25 indicates identity Score > 22 indicates homology |
U | R.KGTDGSQIVTILSNK.G | |
| 4196 |
575.2911 | 1722.8515 | 1722.8399 | 6.71 | 1 | 56 | 9e-006 | 1Score > 27 indicates identity Score > 18 indicates homology |
U | K.NWPIYKDDTTIAMR.K | |
| 4203 |
870.4288 | 1738.8430 | 1738.8348 | 4.72 | 1 | 18 | 0.027 | 1Score > 27 indicates identity Score > 15 indicates homology |
U | K.NWPIYKDDTTIAMR.K + Oxidation (M) | |
| 4204 |
580.6230 | 1738.8472 | 1738.8348 | 7.09 | 1 | 39 | 0.0036 | 1Score > 27 indicates identity |
U | K.NWPIYKDDTTIAMR.K + Oxidation (M) | |
| 4237 |
946.4297 | 1890.8448 | 1890.8339 | 5.78 | 0 | 7 | 1 | 1Score > 26 indicates identity Score > 19 indicates homology |
U | K.STSGSMDDLYNMINTVK.S + Oxidation (M) | |
| 4238 |
946.9294 | 1891.8442 | 1891.8179 | 13.9 | 0 | 26 | 0.0054 | 1Score > 25 indicates identity Score > 16 indicates homology |
U | K.STSGSMDDLYNMINTVK.S + Oxidation (M); Deamidated (NQ) | |
| 4240 |
954.4276 | 1906.8406 | 1906.8288 | 6.19 | 0 | 64 | 8e-006 | 1Score > 25 indicates identity |
U | K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M) | |
| 4241 |
636.6215 | 1906.8427 | 1906.8288 | 7.25 | 0 | 33 | 0.00095 | 1Score > 25 indicates identity Score > 16 indicates homology |
U | K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M) | |
| 4277 |
759.0126 | 2274.0160 | 2273.9859 | 13.2 | 0 | 64 | 1.5e-006 | 1Score > 25 indicates identity Score > 18 indicates homology |
U | K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ) | |
| 4278 |
1138.0155 | 2274.0164 | 2273.9859 | 13.4 | 0 | 68 | 2.3e-006 | 1Score > 25 indicates identity Score > 24 indicates homology |
U | K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ) | |
| 4279 |
759.3420 | 2275.0042 | 2274.9699 | 15.1 | 0 | 7 | 0.3 | 1Score > 25 indicates identity Score > 14 indicates homology |
U | K.SDCPDSTLLGTFVENHDNPR.F + 2 Deamidated (NQ) | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| AMYA1_ASPOR |
675 | 55288 | 1.1 | ||
| Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1 | |||||
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LAPA_ASPOR | 407 | Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UB64_ASPOR | 297 | SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ORYZ_ASPOR | 291 | Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UG98_ASPOR | 286 | Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | P20D2_ASPOR | 282 | Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EGLC_ASPOR | 268 | Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 261 | Trypsin OS=Sus scrofa PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U8Y3_ASPOR | 252 | Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ABNC_ASPOR | 229 | Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1 |
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