MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer16
MS data file : Ofer16.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:12:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,508

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 24 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 35)

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-1

Accession Score Description
1 AMYA3_ASPOR 681 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1
Score Mass Matches Sequences emPAI
1.1 AMYA3_ASPOR 681 55283 33 (25) 17 (15) 3.25
Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

-33 peptide matches (32 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3868   575.3431 574.3358 574.3326 5.57 0 36 0.0096 +1Score > 28 indicates identity U R.IDTVK.H
3916   348.1917 694.3688 694.3650 5.60 0 37 0.0023 +1Score > 23 indicates identity U R.NYAISK.D
3917   695.3767 694.3694 694.3650 6.43 0 19 0.14 +1Score > 23 indicates identity U R.NYAISK.D
3953   410.7242 819.4338 819.4279 7.26 0 14 0.18 +1Score > 27 indicates identity
Score > 20 indicates homology		 U K.NWPIYK.D
3967   849.4766 848.4693 848.4644 5.86 0 17 0.026 +1Score > 25 indicates identity
Score > 14 indicates homology		 U R.VLYPTEK.L
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer16.raw

Score > 25 indicates identity

Score > 23 indicates homology
3968   425.2426 848.4706 848.4644 7.42 0 36 0.0028 -1Score > 25 indicates identity
Score > 23 indicates homology		 U R.VLYPTEK.L
19.1 0 8 1.7 2 VLFEWR  
-19.1 1 8 1.7 2 VLGKYNR  
-19.1 0 8 1.7 2 VLLNHPR   + Deamidated (NQ)
-19.0 1 5 3.3 5 YLLQRR   + Deamidated (NQ)
41.8 1 4 3.8 6 RTQSTEK  
19.9 1 4 4.2 7 VMKSASAR  
46.4 0 3 4.6 8 LVMNNLK   + Oxidation (M); 2 Deamidated (NQ)
7.42 0 3 5.1 9 IVNLFDK   + Deamidated (NQ)
41.8 1 1 7.2 10 RTSQTQK   + Deamidated (NQ)
4005 +1 464.7857 927.5568 927.5501 7.25 0 82 9.7e-008 +1Score > 24 indicates identity U K.LIASANAIR.N
4006   930.4614 929.4541 929.4495 5.01 0 52 0.00016 +1Score > 26 indicates identity U K.DTGFVTYK.N
4007   465.7346 929.4546 929.4495 5.58 0 10 0.45 +1Score > 26 indicates identity
Score > 19 indicates homology		 U K.DTGFVTYK.N
4020   328.5158 982.5256 982.5196 6.12 0 53 7.7e-005 +1Score > 24 indicates identity U K.ALSSALHER.G
4021   492.2709 982.5272 982.5196 7.82 0 76 4.4e-007 +1Score > 24 indicates identity U K.ALSSALHER.G
4038   1026.4741 1025.4668 1025.4607 5.99 0 17 0.32 +1Score > 24 indicates identity U K.DFWPGYNK.A
4039   513.7410 1025.4674 1025.4607 6.60 0 20 0.052 +1Score > 25 indicates identity
Score > 20 indicates homology		 U K.DFWPGYNK.A
4130   657.3445 1312.6744 1312.6663 6.21 0 67 4.8e-006 +1Score > 26 indicates identity U R.FASYTNDIALAK.N
4131   657.8364 1313.6582 1313.6503 6.05 0 95 7.7e-009 +1Score > 27 indicates identity U R.FASYTNDIALAK.N + Deamidated (NQ)
4134   671.8590 1341.7034 1341.6929 7.89 0 62 1.5e-005 +1Score > 27 indicates identity U R.SQSIYFLLTDR.F
4149   716.8906 1431.7666 1431.7569 6.79 0 69 3.1e-006 +1Score > 26 indicates identity
Score > 26 indicates homology		 U K.GTDGSQIVTILSNK.G
4150   478.2631 1431.7675 1431.7569 7.37 0 42 0.00015 +1Score > 26 indicates identity
Score > 16 indicates homology		 U K.GTDGSQIVTILSNK.G
4156   735.8142 1469.6138 1469.6053 5.84 0 24 0.015 +1Score > 22 indicates identity
Score > 18 indicates homology		 U R.TDGSTTATCNTADR.K
4166   506.9244 1517.7514 1517.7415 6.47 1 18 0.07 +1Score > 27 indicates identity
Score > 19 indicates homology		 U K.HVQKDFWPGYNK.A
4172   780.9376 1559.8606 1559.8519 5.62 1 37 0.0031 +1Score > 25 indicates identity U R.KGTDGSQIVTILSNK.G
4173   520.9609 1559.8609 1559.8519 5.76 1 38 0.0017 +1Score > 25 indicates identity
Score > 22 indicates homology		 U R.KGTDGSQIVTILSNK.G
4196   575.2911 1722.8515 1722.8399 6.71 1 56 9e-006 +1Score > 27 indicates identity
Score > 18 indicates homology		 U K.NWPIYKDDTTIAMR.K
4203   870.4288 1738.8430 1738.8348 4.72 1 18 0.027 +1Score > 27 indicates identity
Score > 15 indicates homology		 U K.NWPIYKDDTTIAMR.K + Oxidation (M)
4204   580.6230 1738.8472 1738.8348 7.09 1 39 0.0036 +1Score > 27 indicates identity U K.NWPIYKDDTTIAMR.K + Oxidation (M)
4237   946.4297 1890.8448 1890.8339 5.78 0 7 1 +1Score > 26 indicates identity
Score > 19 indicates homology		 U K.STSGSMDDLYNMINTVK.S + Oxidation (M)
4238   946.9294 1891.8442 1891.8179 13.9 0 26 0.0054 +1Score > 25 indicates identity
Score > 16 indicates homology		 U K.STSGSMDDLYNMINTVK.S + Oxidation (M); Deamidated (NQ)
4240   954.4276 1906.8406 1906.8288 6.19 0 64 8e-006 +1Score > 25 indicates identity U K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M)
4241   636.6215 1906.8427 1906.8288 7.25 0 33 0.00095 +1Score > 25 indicates identity
Score > 16 indicates homology		 U K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M)
4277   759.0126 2274.0160 2273.9859 13.2 0 64 1.5e-006 +1Score > 25 indicates identity
Score > 18 indicates homology		 U K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ)
4278   1138.0155 2274.0164 2273.9859 13.4 0 68 2.3e-006 +1Score > 25 indicates identity
Score > 24 indicates homology		 U K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ)
4279   759.3420 2275.0042 2274.9699 15.1 0 7 0.3 +1Score > 25 indicates identity
Score > 14 indicates homology		 U K.SDCPDSTLLGTFVENHDNPR.F + 2 Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
AMYA1_ASPOR 675 55288 1.1
Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 407 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 Q2UB64_ASPOR 297 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 291 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UG98_ASPOR 286 Glutamate dehydrogenase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000923 PE=3 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 282 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 EGLC_ASPOR 268 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+8

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+9

Accession Score Description
1 Q2U8Y3_ASPOR 252 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+10

Accession Score Description
1 ABNC_ASPOR 229 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
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