MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer6
MS data file : Ofer6.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:09:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,543

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (31 proteins)


Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1.1 AMYA1_ASPOR 750 55288 32 (25) 17 (15) 2.78 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
1.2 AMYA3_ASPOR 704 55283 32 (25) 17 (15) 2.78 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1
2 TRYP_PIG 310 25078 13 (12) 4 (4) 1.72 Trypsin OS=Sus scrofa PE=1 SV=1
3 LAPA_ASPOR 308 41308 10 (9) 10 (9) 1.00 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1
4 ORYZ_ASPOR 297 42546 15 (11) 8 (6) 1.28 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2
5 Q2UB64_ASPOR 244 28038 8 (5) 8 (5) 0.75 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1
6 P20D2_ASPOR 221 45784 11 (7) 11 (7) 0.63 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1
7 ABNC_ASPOR 217 34396 11 (9) 6 (4) 0.90 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
8 RNT2_ASPOR 207 31293 14 (9) 10 (6) 1.48 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2
9 EGLC_ASPOR 166 46742 5 (5) 5 (5) 0.41 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1
10 Q2U2Z5_ASPOR 160 14669 9 (7) 5 (4) 3.31 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
11 Q2ULW8_ASPOR 151 25695 5 (5) 4 (4) 0.84 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1
12 Q2UTD2_ASPOR 107 33809 8 (5) 7 (4) 0.60 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1
13 Q2U8Y3_ASPOR 90 31694 3 (3) 3 (3) 0.35 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1
14 Q2U9Z9_ASPOR 50 59229 2 (1) 2 (1) 0.06 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000599 PE=3 SV=1
15 LECF_ASPOR 44 34690 2 (1) 2 (1) 0.10 Fucose-specific lectin OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=fleA PE=1 SV=1
16 Q2UU21_ASPOR 35 31604 4 (2) 3 (2) 0.22 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000493 PE=4 SV=1
17 Q2U7H4_ASPOR 34 32304 1 (1) 1 (1) 0.10 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000838 PE=3 SV=1
18 Q2UF44_ASPOR 33 78261 3 (1) 2 (1) 0.04 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090026000355 PE=4 SV=1
19 Q2U5F1_ASPOR 30 34394 1 (1) 1 (1) 0.10 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090113000169 PE=4 SV=1
20 Q2TXV6_ASPOR 30 24577 1 (1) 1 (1) 0.14 FAD_binding_2 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090103000503 PE=4 SV=1
21 GFP_AEQVI 29 26983 3 (1) 3 (1) 0.12 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1
22 Q2U0B2_ASPOR 27 37186 2 (1) 2 (1) 0.09 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000510 PE=4 SV=1
23 AMYG_ASPOR 25 65929 2 (1) 2 (1) 0.05 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2
24 Q2U287_ASPOR 24 59365 1 (1) 1 (1) 0.06 UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1
25 Q2TW93_ASPOR 21 19014 1 (1) 1 (1) 0.18 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090010000651 PE=4 SV=1
26 Q2TY02_ASPOR 19 139522 2 (1) 1 (1) 0.02 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090103000443 PE=4 SV=1
27 Q2UIL4_ASPOR 19 47620 1 (1) 1 (1) 0.07 Amine oxidase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000011 PE=3 SV=1
28 Q2USC5_ASPOR 18 70722 1 (1) 1 (1) 0.05 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000482 PE=3 SV=1
29 Q2PIQ7_ASPOR 16 37147 3 (1) 1 (1) 0.09 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090206000108 PE=4 SV=1
30 Q2U2D8_ASPOR 16 40878 1 (1) 1 (1) 0.08 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000493 PE=4 SV=1

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