MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer6
MS data file : Ofer6.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:09:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,543

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 30)

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+1

Accession Score Description
Family member distances as a dendrogram 1 AMYA1_ASPOR 750 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
2 AMYA3_ASPOR 704 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 TRYP_PIG 310 Trypsin OS=Sus scrofa PE=1 SV=1

+3

Accession Score Description
1 LAPA_ASPOR 308 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 297 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UB64_ASPOR 244 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 221 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 ABNC_ASPOR 217 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

-8

Accession Score Description
1 RNT2_ASPOR 207 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2
Score Mass Matches Sequences emPAI
8.1 RNT2_ASPOR 207 31293 14 (9) 10 (6) 1.48
Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

-14 peptide matches (14 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3640   682.3447 681.3374 681.3333 5.99 0 22 0.085 +1Score > 24 indicates identity
Score > 24 indicates homology		 U K.GLDSYK.A
3641   341.6760 681.3374 681.3333 6.02 0 16 0.4 +1Score > 24 indicates identity U K.GLDSYK.A
3659   722.4128 721.4055 721.4010 6.21 0 39 0.0019 +1Score > 24 indicates identity U K.TVDLFK.G
3660   361.7103 721.4060 721.4010 6.93 0 31 0.011 +1Score > 24 indicates identity U K.TVDLFK.G
3662   369.1788 736.3430 736.3392 5.28 0 13 0.85 +1Score > 25 indicates identity U K.DYSPQK.E
3729   437.2401 872.4656 872.4603 6.08 0 43 0.0017 +1Score > 28 indicates identity U K.AGIVPDSSK.T
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer6.raw

Score > 27 indicates identity
3752   476.2455 950.4764 950.4709 5.84 0 26 0.068 -1Score > 27 indicates identity U K.EVGDYLQK.T
-13.7 1 17 0.52 2 DIYGMPKK  
44.1 0 12 1.8 3 GDLDEFGAK  
5.84 0 12 1.9 4 EQFQTLGK   + Deamidated (NQ)
44.1 0 8 4.4 5 DINGAFDAK   + Deamidated (NQ)
-32.4 1 7 5.1 6 NALTFEKK   + Deamidated (NQ)
12.7 0 7 5.3 7 DLGFNMVR  
-5.95 1 7 5.7 8 NYERIGAK   + Deamidated (NQ)
-29.6 1 6 6.8 9 VQRGHPTR   + Deamidated (NQ)
5.84 0 5 8.1 10 EVEQTFAK  
3765   492.7597 983.5048 983.4997 5.19 0 61 7.3e-006 +1Score > 25 indicates identity
Score > 22 indicates homology		 U R.TELLSYMK.K
3768   500.7577 999.5008 999.4947 6.19 0 38 0.0026 +1Score > 26 indicates identity
Score > 24 indicates homology		 U R.TELLSYMK.K + Oxidation (M)
3797   376.8729 1127.5969 1127.5896 6.44 1 10 1 +1Score > 26 indicates identity
Score > 22 indicates homology		 U R.TELLSYMKK.Y + Oxidation (M)
3850   480.9185 1439.7337 1439.7256 5.62 0 43 9.5e-005 +1Score > 26 indicates identity
Score > 16 indicates homology		 U R.SEIESALAAIHDGK.K
3880   840.3843 1678.7540 1678.7443 5.79 0 21 0.015 +1Score > 26 indicates identity
Score > 16 indicates homology		 U K.HGTCINTIEPSCYK.D
3881   560.5928 1678.7566 1678.7443 7.30 0 59 3e-005 +1Score > 26 indicates identity U K.HGTCINTIEPSCYK.D
4117   856.7537 2567.2393 2567.2061 12.9 0 47 7.1e-005 +1Score > 27 indicates identity
Score > 18 indicates homology		 U K.GNAITGEYQPIDTLTSPGCSTSGIK.Y + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q2UDP7_ASPOR 39 201276 8.1
AAA domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090012000087 PE=4 SV=1

+9

Accession Score Description
1 EGLC_ASPOR 166 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+10

Accession Score Description
1 Q2U2Z5_ASPOR 160 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
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