MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer6
MS data file : Ofer6.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:09:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,543

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 30)

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+1

Accession Score Description
Family member distances as a dendrogram 1 AMYA1_ASPOR 750 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
2 AMYA3_ASPOR 704 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 TRYP_PIG 310 Trypsin OS=Sus scrofa PE=1 SV=1

+3

Accession Score Description
1 LAPA_ASPOR 308 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 297 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UB64_ASPOR 244 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

-6

Accession Score Description
1 P20D2_ASPOR 221 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1
Score Mass Matches Sequences emPAI
6.1 P20D2_ASPOR 221 45784 11 (7) 11 (7) 0.63
Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

-11 peptide matches (11 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3613   315.6835 629.3524 629.3497 4.41 0 3 1.7 +7Score > 28 indicates identity
Score > 18 indicates homology		 U R.ADVAVR.L
3741   457.2797 912.5448 912.5392 6.16 0 48 0.00019 +1Score > 23 indicates identity U K.LIEAALQR.R
3769   500.7595 999.5044 999.4985 5.95 0 61 1.7e-005 +1Score > 26 indicates identity U R.LAAGTPDEAR.D
3777   521.3278 1040.6410 1040.6342 6.59 1 30 0.0065 +1Score > 20 indicates identity U R.KLIEAALQR.R
3803   585.3151 1168.6156 1168.6088 5.86 0 16 0.37 +1Score > 26 indicates identity
Score > 24 indicates homology		 U R.VDQLGNIPADK.G
3804   592.8572 1183.6998 1183.6924 6.26 0 40 0.00093 +1Score > 22 indicates identity U R.SAISAVLPALSR.V
3842   470.2361 1407.6865 1407.6796 4.87 0 24 0.11 +1Score > 27 indicates identity
Score > 27 indicates homology		 U K.GHAAHSGYPWLGR.S
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer6.raw

Score > 25 indicates identity
3843   705.8934 1409.7722 1409.7627 6.79 0 79 2.1e-007 -1Score > 25 indicates identity U R.VDAGVAANVVPATAR.A
-19.0 1 12 1 2 RNAVLSPPSLLSR   + Deamidated (NQ)
-11.0 1 8 2.6 3 ADVVKALNINPEK  
3.94 0 5 5.1 4 ENVVSQLLPFHK  
14.8 0 3 8.1 5 INNVVAQQEGPIK   + Deamidated (NQ)
14.8 0 3 8.1 5 INNVVAQQEGPIK   + Deamidated (NQ)
-16.3 0 3 8.5 7 QIAAQPMLLLPAK   + Oxidation (M); Deamidated (NQ)
-36.9 0 2 11 8 DALTIALVVDKPR  
-36.8 0 2 12 9 LATLSQLNLLAPR   + Deamidated (NQ)
24.6 1 1 14 10 VNAETVAHGRISR   + Deamidated (NQ)
3856   495.2690 1482.7852 1482.7790 4.14 1 22 0.069 +1Score > 26 indicates identity
Score > 23 indicates homology		 U R.LAAGTPDEARDIVR.R
3911   892.8941 1783.7736 1783.7649 4.89 0 50 2.2e-005 +1Score > 25 indicates identity
Score > 16 indicates homology		 U R.DATDGNPDVYAEFNTR.S
3941   632.0125 1893.0157 1892.9843 16.5 1 40 0.00024 +1Score > 25 indicates identity
Score > 16 indicates homology		 U R.VDQLGNIPADKGGLPSSPK.Y + Deamidated (NQ)

+7

Accession Score Description
1 ABNC_ASPOR 217 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

+8

Accession Score Description
1 RNT2_ASPOR 207 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

+9

Accession Score Description
1 EGLC_ASPOR 166 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+10

Accession Score Description
1 Q2U2Z5_ASPOR 160 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
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