MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Ofer6 |
| MS data file | : | Ofer6.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 7 May 2026 at 18:09:20 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,543 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMYA1_ASPOR | 750 | Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1 | |
| 2 | AMYA3_ASPOR | 704 | Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 310 | Trypsin OS=Sus scrofa PE=1 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LAPA_ASPOR | 308 | Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ORYZ_ASPOR | 297 | Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UB64_ASPOR | 244 | SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 5.1 | Q2UB64_ASPOR |
244 | 28038 | 8 (5) | 8 (5) | 0.75 | |
| SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1 | |||||||
8 peptide matches (8 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3669 |
374.1871 | 746.3596 | 746.3559 | 5.09 | 0 | 12 | 1.2 | 5Score > 28 indicates identity Score > 25 indicates homology |
U | R.SLENER.V | |
| 3685 |
394.7316 | 787.4486 | 787.4440 | 5.96 | 0 | 20 | 0.15 | 1Score > 27 indicates identity Score > 24 indicates homology |
U | K.LQTALDK.G | |
| 3784 |
538.2779 | 1074.5412 | 1074.5346 | 6.21 | 0 | 83 | 1.6e-007 | 1Score > 28 indicates identity |
U | R.AGGDWDTLLK.K | |
| 3795 |
555.2846 | 1108.5546 | 1108.5513 | 3.05 | 0 | 71 | 2e-006 | 1Score > 26 indicates identity |
U | K.TGASYIDLNR.A | |
| 3816 |
619.3352 | 1236.6558 | 1236.6462 | 7.78 | 1 | 3 | 1 | 1Score > 26 indicates identity Score > 16 indicates homology |
U | K.KTGASYIDLNR.A | |
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer6.rawScore > 22 indicates identity |
|||||||||||
| 3838 |
690.4078 | 1378.8010 | 1378.7933 | 5.65 | 0 | 85 | 2.8e-008 | 1Score > 22 indicates identity |
U | R.NAGGVPVLVTPLSR.R | |
| 48.4 | 0 | 21 | 0.072 | 2 | VAVEPVNPSELPK + Deamidated (NQ) | ||||||
| 0.29 | 0 | 3 | 4.4 | 3 | MSPIVQLVLHVK + Oxidation (M) | ||||||
| 32.1 | 1 | 0 | 8.1 | 4 | LREVAPNVEAGPK | ||||||
| 3868 |
537.2548 | 1608.7426 | 1608.7281 | 9.00 | 0 | 68 | 8.9e-007 | 1Score > 26 indicates identity Score > 20 indicates homology |
U | K.GQNYGHNGATTVSFR.A + Deamidated (NQ) | |
| 4004 |
728.3707 | 2182.0903 | 2182.0616 | 13.1 | 0 | 26 | 0.0042 | 1Score > 27 indicates identity Score > 15 indicates homology |
U | R.ASTVYLNSIGPVSAHMYDLK.T + Oxidation (M); Deamidated (NQ) | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | P20D2_ASPOR | 221 | Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ABNC_ASPOR | 217 | Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RNT2_ASPOR | 207 | Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EGLC_ASPOR | 166 | Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U2Z5_ASPOR | 160 | Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1 |
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