MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer6
MS data file : Ofer6.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:09:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,543

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 30)


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+1

Accession Score Description
Family member distances as a dendrogram 1 AMYA1_ASPOR 750 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
2 AMYA3_ASPOR 704 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

+2

Accession Score Description
1 TRYP_PIG 310 Trypsin OS=Sus scrofa PE=1 SV=1

-3

Accession Score Description
1 LAPA_ASPOR 308 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1
Score Mass Matches Sequences emPAI
3.1 LAPA_ASPOR 308 41308 10 (9) 10 (9) 1.00
Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

-10 peptide matches (10 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3698   409.2393 816.4640 816.4593 5.85 0 33 0.012 +1Score > 27 indicates identity U R.VLLTDEK.V
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer6.raw

Score > 27 indicates identity

Score > 26 indicates homology

3731   438.7471 875.4796 875.4753 4.99 0 48 0.00043 -1Score > 27 indicates identity
Score > 26 indicates homology
U K.GVTVEPFK.H
29.9 0 11 2 2 QMVQGIGK   + Oxidation (M)
-11.7 1 10 2.3 3 RVTIMQK   + Deamidated (NQ)
42.7 1 10 2.7 4 KQPMEVK   + Oxidation (M); Deamidated (NQ)
-49.4 1 8 3.6 5 QFVKLNK  
-7.84 0 8 3.8 6 VGQAFVQK  
-20.6 1 7 4.8 7 RFAAINGK  
-49.4 1 7 4.9 8 RDFVVLK  
-7.82 1 7 4.9 8 RDLTFPK  
-36.5 0 7 5 10 EQVLIFK  
3748   467.7385 933.4624 933.4556 7.36 0 58 4.3e-005 +1Score > 27 indicates identity U K.SSEWLQGK.I
3796   558.8198 1115.6250 1115.6186 5.77 0 61 2e-005 +1Score > 26 indicates identity U K.IQEIISASGAK.G
3813   607.8287 1213.6428 1213.6455 -2.19 0 22 0.17 +1Score > 26 indicates identity U K.HSFPQSSLIAK.I
3844   709.8226 1417.6306 1417.6218 6.28 0 53 3.2e-005 +1Score > 25 indicates identity
Score > 21 indicates homology
U K.AMLQQDMTGYTK.G + 2 Oxidation (M)
3882   840.9338 1679.8530 1679.8440 5.36 0 78 8.3e-008 +1Score > 27 indicates identity
Score > 20 indicates homology
U K.VIVDAYCTIPTVDSK.C
3932   622.6426 1864.9060 1864.8744 16.9 0 53 0.00015 +1Score > 27 indicates identity U K.NFETVLQPFSEFHNR.Y + Deamidated (NQ)
3979   704.0282 2109.0628 2109.0338 13.7 0 52 3.3e-005 +1Score > 27 indicates identity
Score > 19 indicates homology
U K.TIVLGAHQDSINLDSPSEGR.A + Deamidated (NQ)
4088   830.4238 2488.2496 2488.2180 12.7 0 61 3.1e-006 +1Score > 27 indicates identity
Score > 18 indicates homology
U K.GTTDAGKPESIGIITDNVDENLTK.F + Deamidated (NQ)

+4

Accession Score Description
1 ORYZ_ASPOR 297 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UB64_ASPOR 244 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 221 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 ABNC_ASPOR 217 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

+8

Accession Score Description
1 RNT2_ASPOR 207 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

+9

Accession Score Description
1 EGLC_ASPOR 166 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+10

Accession Score Description
1 Q2U2Z5_ASPOR 160 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
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