MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer6
MS data file : Ofer6.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:09:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,543

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 30)

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-21

Accession Score Description
1 GFP_AEQVI 29 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1
Score Mass Matches Sequences emPAI
21.1 GFP_AEQVI 29 26983 3 (1) 3 (1) 0.12
Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1
2 samesets of GFP_AEQVI
IGF1_BOVIN 29 42206 3 (1) 3 (1) 0.08
Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal
B7UCZ6_9RHAB 29 85329 3 (1) 3 (1) 0.04
G protein/GFP fusion protein OS=Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP OX=582817 GN=G PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3703   413.7125 825.4104 825.4055 6.01 0 24 0.056 +1Score > 24 indicates identity U K.FICTTGK.L
3829   641.7949 1281.5752 1281.5659 7.27 0 29 0.011 +1Score > 26 indicates identity
Score > 22 indicates homology		 U K.SAMPEGYVQER.T + Oxidation (M)
3836   449.8933 1346.6581 1346.6507 5.50 1 10 0.22 +1Score > 27 indicates identity
Score > 15 indicates homology		 U R.TIFFKDDGNYK.T

+22

Accession Score Description
1 Q2U0B2_ASPOR 27 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000510 PE=4 SV=1

+23

Accession Score Description
1 AMYG_ASPOR 25 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

+24

Accession Score Description
1 Q2U287_ASPOR 24 UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1

+25

Accession Score Description
1 Q2TW93_ASPOR 21 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090010000651 PE=4 SV=1

+26

Accession Score Description
1 Q2TY02_ASPOR 19 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090103000443 PE=4 SV=1

+27

Accession Score Description
1 Q2UIL4_ASPOR 19 Amine oxidase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000011 PE=3 SV=1

+28

Accession Score Description
1 Q2USC5_ASPOR 18 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000482 PE=3 SV=1

+29

Accession Score Description
1 Q2PIQ7_ASPOR 16 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090206000108 PE=4 SV=1

+30

Accession Score Description
1 Q2U2D8_ASPOR 16 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000493 PE=4 SV=1
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