MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer6
MS data file : Ofer6.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:09:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,543

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 30)

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-1

Accession Score Description
Family member distances as a dendrogram 1 AMYA1_ASPOR 750 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
2 AMYA3_ASPOR 704 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
AMYA1_ASPOR 750 55288 32 (25) 17 (15) 2.78
Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
AMYA3_ASPOR 704 55283 32 (25) 17 (15) 2.78
Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

-33 peptide matches (29 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
3580   575.3420 574.3347 574.3326 3.65 0 33 0.021 +1Score > 28 indicates identity U X X R.IDTVK.H
3646   348.1912 694.3678 694.3650 4.16 0 36 0.0024 +1Score > 23 indicates identity U X X R.NYAISK.D
3647   695.3759 694.3686 694.3650 5.28 0 25 0.031 +1Score > 23 indicates identity U X X R.NYAISK.D
3700   410.7240 819.4334 819.4279 6.77 0 11 0.34 +1Score > 27 indicates identity
Score > 19 indicates homology		 U X X K.NWPIYK.D
3716   849.4769 848.4696 848.4644 6.21 0 18 0.29 +1Score > 25 indicates identity U X X R.VLYPTEK.L
3717   425.2423 848.4700 848.4644 6.71 0 48 0.00021 +1Score > 25 indicates identity
Score > 24 indicates homology		 U X X R.VLYPTEK.L
3745 +1 464.7856 927.5566 927.5501 7.03 0 59 1.9e-005 +1Score > 24 indicates identity U X X K.LIASANAIR.N
3746   930.4612 929.4539 929.4495 4.80 0 58 2.6e-005 +1Score > 26 indicates identity
Score > 25 indicates homology		 U X X K.DTGFVTYK.N
3747   465.7349 929.4552 929.4495 6.22 0 23 0.057 +1Score > 26 indicates identity
Score > 23 indicates homology		 U X X K.DTGFVTYK.N
3763 +1 328.5157 982.5253 982.5196 5.81 0 56 3.8e-005 +1Score > 24 indicates identity U X X K.ALSSALHER.G
3764   492.2706 982.5266 982.5196 7.21 0 71 1.2e-006 +1Score > 24 indicates identity U X X K.ALSSALHER.G
3773   1026.4725 1025.4652 1025.4607 4.43 0 11 0.14 +1Score > 24 indicates identity
Score > 15 indicates homology		 U X X K.DFWPGYNK.A
3774   513.7404 1025.4662 1025.4607 5.43 0 19 0.041 +1Score > 24 indicates identity
Score > 18 indicates homology		 U X X K.DFWPGYNK.A
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer6.raw

Score > 27 indicates identity

Score > 23 indicates homology
3832 +1 657.8362 1313.6578 1313.6503 5.75 0 76 3.3e-007 -1Score > 27 indicates identity
Score > 23 indicates homology		 U X X R.FASYTNDIALAK.N + Deamidated (NQ)
35.5 1 6 2.9 2 FASDAPEHRER  
13.8 0 6 2.9 2 MASSHLSELPAR   + Oxidation (M)
-41.7 0 6 3 4 MAGLPTAVQVVGR   + Oxidation (M)
-8.97 1 5 3.9 5 MAHSVTMRLPR   + Oxidation (M)
16.7 0 3 6.1 6 MTGLGMETIFAK   + Oxidation (M)
44.4 0 3 6.5 7 FAQMMLEATQK   + Oxidation (M); Deamidated (NQ)
44.4 0 0 11 8 FAQMMLEATQK   + Oxidation (M); Deamidated (NQ)
3847   716.8893 1431.7640 1431.7569 4.97 0 106 6.8e-010 +1Score > 26 indicates identity U X X K.GTDGSQIVTILSNK.G
3848   478.2631 1431.7675 1431.7569 7.37 0 27 0.0047 +1Score > 26 indicates identity
Score > 16 indicates homology		 U X X K.GTDGSQIVTILSNK.G
3854   735.8135 1469.6124 1469.6053 4.88 0 29 0.013 +1Score > 22 indicates identity U X R.TDGSTTATCNTADR.K
3858   506.9239 1517.7499 1517.7415 5.49 1 18 0.06 +1Score > 27 indicates identity
Score > 19 indicates homology		 U X X K.HVQKDFWPGYNK.A
3862   780.9371 1559.8596 1559.8519 4.98 1 60 1.9e-005 +1Score > 25 indicates identity U X X R.KGTDGSQIVTILSNK.G
3863   521.2949 1560.8629 1560.8359 17.3 1 32 0.002 +1Score > 25 indicates identity
Score > 18 indicates homology		 U X X R.KGTDGSQIVTILSNK.G + Deamidated (NQ)
3867   785.8394 1569.6642 1569.6577 4.17 0 77 2.9e-007 +1Score > 24 indicates identity U X R.TDGSTTATCNTADQK.Y
3887   575.2910 1722.8512 1722.8399 6.53 1 50 2e-005 +1Score > 27 indicates identity
Score > 16 indicates homology		 U X X K.NWPIYKDDTTIAMR.K
3892   870.4288 1738.8430 1738.8348 4.72 1 20 0.027 +1Score > 27 indicates identity
Score > 17 indicates homology		 U X X K.NWPIYKDDTTIAMR.K + Oxidation (M)
3893   580.6224 1738.8454 1738.8348 6.06 1 38 0.0013 +1Score > 27 indicates identity
Score > 22 indicates homology		 U X X K.NWPIYKDDTTIAMR.K + Oxidation (M)
3931   930.4429 1858.8712 1858.8625 4.71 0 50 0.00014 +1Score > 27 indicates identity
Score > 24 indicates homology		 U X X R.EATWLSGYPTDSELYK.L
3945   954.4274 1906.8402 1906.8288 5.98 0 76 8.5e-008 +1Score > 25 indicates identity
Score > 17 indicates homology		 U X X K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M)
3946   636.9551 1907.8435 1907.8129 16.1 0 22 0.095 +1Score > 25 indicates identity U X X K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M); Deamidated (NQ)
4030   1138.0146 2274.0146 2273.9859 12.6 0 44 0.00018 +1Score > 25 indicates identity
Score > 19 indicates homology		 U X X K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ)
4031 +1 759.0128 2274.0166 2273.9859 13.5 0 62 1.8e-006 +1Score > 25 indicates identity
Score > 18 indicates homology		 U X X K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ)

+2

Accession Score Description
1 TRYP_PIG 310 Trypsin OS=Sus scrofa PE=1 SV=1

+3

Accession Score Description
1 LAPA_ASPOR 308 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 297 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UB64_ASPOR 244 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 221 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 ABNC_ASPOR 217 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

+8

Accession Score Description
1 RNT2_ASPOR 207 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

+9

Accession Score Description
1 EGLC_ASPOR 166 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+10

Accession Score Description
1 Q2U2Z5_ASPOR 160 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
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