MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer6
MS data file : Ofer6.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:09:20 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,543

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 30)

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+1

Accession Score Description
Family member distances as a dendrogram 1 AMYA1_ASPOR 750 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
2 AMYA3_ASPOR 704 Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

-2

Accession Score Description
1 TRYP_PIG 310 Trypsin OS=Sus scrofa PE=1 SV=1
Score Mass Matches Sequences emPAI
2.1 TRYP_PIG 310 25078 13 (12) 4 (4) 1.72
Trypsin OS=Sus scrofa PE=1 SV=1

-13 peptide matches (9 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3707   842.5127 841.5054 841.5022 3.89 0 10 0.45 +1Score > 24 indicates identity
Score > 20 indicates homology		 U R.VATVSLPR.S
3710 +3 421.7609 841.5072 841.5022 6.06 0 50 0.00012 +1Score > 24 indicates identity U R.VATVSLPR.S
3779 -1 523.2892 1044.5638 1044.5564 7.18 0 54 0.00012 +1Score > 27 indicates identity U K.LSSPATLNSR.V
3778   523.2891 1044.5636 1044.5564 6.99 0 (53) 4.1e-005 +1Score > 27 indicates identity
Score > 22 indicates homology		 U K.LSSPATLNSR.V
4015   1106.5618 2211.1090 2211.0807 12.8 0 49 3.6e-005 +1Score > 27 indicates identity
Score > 17 indicates homology		 U R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ)
4016   738.0441 2211.1105 2211.0807 13.4 0 58 8e-006 +1Score > 27 indicates identity
Score > 20 indicates homology		 U R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ)
4035   762.0708 2283.1906 2283.1569 14.8 0 50 0.00021 +1Score > 26 indicates identity U K.IITHPNFNGNTLDNDIMLIK.L + Deamidated (NQ)
4036   762.3981 2284.1725 2284.1409 13.8 0 43 0.00013 +1Score > 26 indicates identity
Score > 17 indicates homology		 U K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ)
4049   767.4017 2299.1833 2299.1518 13.7 0 35 0.0078 +1Score > 26 indicates identity U K.IITHPNFNGNTLDNDIMLIK.L + Oxidation (M); Deamidated (NQ)
4050   767.7299 2300.1679 2300.1358 13.9 0 46 6.2e-005 +1Score > 27 indicates identity
Score > 16 indicates homology		 U K.IITHPNFNGNTLDNDIMLIK.L + Oxidation (M); 2 Deamidated (NQ)

+3

Accession Score Description
1 LAPA_ASPOR 308 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 297 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2UB64_ASPOR 244 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+6

Accession Score Description
1 P20D2_ASPOR 221 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+7

Accession Score Description
1 ABNC_ASPOR 217 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

+8

Accession Score Description
1 RNT2_ASPOR 207 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

+9

Accession Score Description
1 EGLC_ASPOR 166 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+10

Accession Score Description
1 Q2U2Z5_ASPOR 160 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
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