MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer4
MS data file : Ofer4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:07:42 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,499

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 17)


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+1

Accession Score Description
1 AMYA1_ASPOR 586 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 289 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 P20D2_ASPOR 190 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 189 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2U8Y3_ASPOR 186 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+6

Accession Score Description
1 Q2UB64_ASPOR 180 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+7

Accession Score Description
1 Q2ULW8_ASPOR 174 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1

-8

Accession Score Description
1 EGLC_ASPOR 161 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1
Score Mass Matches Sequences emPAI
8.1 EGLC_ASPOR 161 46742 5 (5) 5 (5) 0.41
Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4049   429.2146 856.4146 856.4079 7.86 0 28 0.004 +1Score > 24 indicates identity
Score > 17 indicates homology
U K.GFNYGATK.S
4082   491.2511 980.4876 980.4815 6.29 0 42 0.0014 +1Score > 26 indicates identity U K.SLFDDSVAK.T
4131   605.3189 1208.6232 1208.6150 6.86 0 40 0.0014 +1Score > 26 indicates identity
Score > 24 indicates homology
U K.NLVGTSGFTSAR.L
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer4.raw

Score > 27 indicates identity

4143   637.3110 1272.6074 1272.5986 6.94 0 67 4.5e-006 -1Score > 27 indicates identity U K.SAISQYGDSFAK.L
-1.87 0 16 0.71 2 ASSIYQHNVPR   + 2 Deamidated (NQ)
-13.9 1 8 3.8 3 DVEAWWKSPR  
12.1 1 5 7.4 4 GNRTGAMGYFAK   + Deamidated (NQ)
1.12 0 4 11 5 ASILFDFGEMK   + Oxidation (M)
12.0 0 4 11 5 TGHMPFVSQPR   + Oxidation (M); Deamidated (NQ)
-42.5 0 2 17 7 FELIHFTNPR  
-41.9 1 2 17 7 MVEAARLSQPR   + Oxidation (M)
1.27 1 2 17 7 RNQSVDTHSTK   + Deamidated (NQ)
24.1 1 1 21 10 DRSMPNAPSGPK   + Oxidation (M); Deamidated (NQ)
4165   731.8243 1461.6340 1461.6260 5.53 0 73 2.1e-007 +1Score > 24 indicates identity
Score > 19 indicates homology
U K.SQSDFESEFSTAK.N

+9

Accession Score Description
1 TRYP_PIG 138 Trypsin OS=Sus scrofa PE=1 SV=1

+10

Accession Score Description
1 Q2U2Z5_ASPOR 107 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
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