MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer4
MS data file : Ofer4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:07:42 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,499

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 17)

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+1

Accession Score Description
1 AMYA1_ASPOR 586 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 289 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 P20D2_ASPOR 190 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

-4

Accession Score Description
1 ORYZ_ASPOR 189 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2
Score Mass Matches Sequences emPAI
4.1 ORYZ_ASPOR 189 42546 13 (8) 8 (6) 0.82
Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

-13 peptide matches (13 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3983   326.6892 651.3638 651.3592 7.20 0 22 0.075 +1Score > 24 indicates identity U K.TYGIAK.K
3984   652.3716 651.3643 651.3592 7.93 0 21 0.03 +1Score > 24 indicates identity
Score > 18 indicates homology		 U K.TYGIAK.K
4008   359.2310 716.4474 716.4432 5.90 0 23 0.057 +1Score > 23 indicates identity U K.ASILSVK.V
4043   423.2794 844.5442 844.5382 7.19 1 36 0.00051 +1Score > 16 indicates identity U K.KASILSVK.V
4047   429.2141 856.4136 856.4079 6.70 0 48 4.9e-005 +1Score > 24 indicates identity
Score > 18 indicates homology		 U R.ASFSNFGK.V
4048   857.4210 856.4137 856.4079 6.79 0 10 1 +1Score > 24 indicates identity
Score > 22 indicates homology		 U R.ASFSNFGK.V
4129   596.2908 1190.5670 1190.5567 8.66 0 6 1.5 +4Score > 27 indicates identity
Score > 20 indicates homology		 U K.GSPNLLAYNGNA.- + Deamidated (NQ)
4135   413.8906 1238.6500 1238.6408 7.44 0 52 0.00016 +1Score > 26 indicates identity U K.SAPWGLGSISHK.G
4136   620.3325 1238.6504 1238.6408 7.82 0 52 9.7e-005 +1Score > 26 indicates identity
Score > 25 indicates homology		 U K.SAPWGLGSISHK.G
4145   642.8213 1283.6280 1283.6180 7.85 0 74 8.1e-007 +1Score > 26 indicates identity
Score > 26 indicates homology		 U K.AAINMSLGGGYSK.A + Oxidation (M)
4146   643.3323 1284.6500 1284.6020 37.4 0 28 0.0031 +1Score > 27 indicates identity
Score > 15 indicates homology		 U K.AAINMSLGGGYSK.A + Oxidation (M); Deamidated (NQ)
4291   627.3173 2505.2401 2505.1997 16.1 0 7 1 +1Score > 27 indicates identity
Score > 20 indicates homology		 U K.AYNAAGGQHVDSIGHGTHVSGTIAGK.T + Deamidated (NQ)
4292   502.0553 2505.2401 2505.1997 16.1 0 23 0.13 +1Score > 27 indicates identity U K.AYNAAGGQHVDSIGHGTHVSGTIAGK.T + Deamidated (NQ)

+5

Accession Score Description
1 Q2U8Y3_ASPOR 186 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+6

Accession Score Description
1 Q2UB64_ASPOR 180 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+7

Accession Score Description
1 Q2ULW8_ASPOR 174 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1

+8

Accession Score Description
1 EGLC_ASPOR 161 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+9

Accession Score Description
1 TRYP_PIG 138 Trypsin OS=Sus scrofa PE=1 SV=1

+10

Accession Score Description
1 Q2U2Z5_ASPOR 107 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
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