MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Ofer4 |
| MS data file | : | Ofer4.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 7 May 2026 at 18:07:42 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,499 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMYA1_ASPOR | 586 | Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LAPA_ASPOR | 289 | Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | P20D2_ASPOR | 190 | Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 3.1 | P20D2_ASPOR |
190 | 45784 | 8 (8) | 8 (8) | 0.75 | |
| Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1 | |||||||
8 peptide matches (8 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 4063 |
457.2799 | 912.5452 | 912.5392 | 6.59 | 0 | 48 | 0.00021 | 1Score > 23 indicates identity |
U | K.LIEAALQR.R | |
| 4089 |
500.7608 | 999.5070 | 999.4985 | 8.55 | 0 | 60 | 2.1e-005 | 1Score > 26 indicates identity |
U | R.LAAGTPDEAR.D | |
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer4.rawScore > 20 indicates identity |
|||||||||||
| 4099 |
521.3281 | 1040.6416 | 1040.6342 | 7.17 | 1 | 40 | 0.00065 | 1Score > 20 indicates identity |
U | R.KLIEAALQR.R | |
| -17.0 | 1 | 18 | 0.095 | 2 | LKIENAILK | ||||||
| 42.1 | 0 | 15 | 0.21 | 3 | QLQALLAER | ||||||
| 7.16 | 0 | 11 | 0.52 | 4 | QLAINLTLR | ||||||
| 7.14 | 0 | 11 | 0.52 | 4 | QLTVLQLAR | ||||||
| 42.1 | 1 | 10 | 0.68 | 6 | KPETPVRSK | ||||||
| 7.16 | 0 | 4 | 2.6 | 7 | LGAINTLIAR | ||||||
| 42.1 | 1 | 4 | 2.6 | 7 | SPGLRLAQAK + Deamidated (NQ) | ||||||
| 4126 |
585.3151 | 1168.6156 | 1168.6088 | 5.86 | 0 | 19 | 0.029 | 1Score > 26 indicates identity Score > 16 indicates homology |
U | R.VDQLGNIPADK.G | |
| 4128 |
592.8577 | 1183.7008 | 1183.6924 | 7.10 | 0 | 36 | 0.0022 | 1Score > 22 indicates identity |
U | R.SAISAVLPALSR.V | |
| 4158 |
705.8939 | 1409.7732 | 1409.7627 | 7.50 | 0 | 67 | 2.9e-006 | 1Score > 25 indicates identity Score > 25 indicates homology |
U | R.VDAGVAANVVPATAR.A | |
| 4207 |
892.8953 | 1783.7760 | 1783.7649 | 6.24 | 0 | 36 | 0.0043 | 1Score > 25 indicates identity |
U | R.DATDGNPDVYAEFNTR.S | |
| 4229 |
632.0135 | 1893.0187 | 1892.9843 | 18.1 | 1 | 35 | 0.00063 | 1Score > 25 indicates identity Score > 16 indicates homology |
U | R.VDQLGNIPADKGGLPSSPK.Y + Deamidated (NQ) | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ORYZ_ASPOR | 189 | Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U8Y3_ASPOR | 186 | Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UB64_ASPOR | 180 | SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2ULW8_ASPOR | 174 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EGLC_ASPOR | 161 | Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 138 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U2Z5_ASPOR | 107 | Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1 |
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