MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Ofer4 |
| MS data file | : | Ofer4.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 7 May 2026 at 18:07:42 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,499 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMYA1_ASPOR | 586 | Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LAPA_ASPOR | 289 | Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 2.1 | LAPA_ASPOR |
289 | 41308 | 8 (8) | 8 (8) | 0.85 | |
| Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 | |||||||
8 peptide matches (8 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 4030 |
409.2404 | 816.4662 | 816.4593 | 8.54 | 0 | 48 | 0.00041 | 1Score > 26 indicates identity |
U | R.VLLTDEK.V | |
| 4068 |
467.7391 | 933.4636 | 933.4556 | 8.64 | 0 | 58 | 4.3e-005 | 1Score > 27 indicates identity |
U | K.SSEWLQGK.I | |
| 4120 |
558.8203 | 1115.6260 | 1115.6186 | 6.67 | 0 | 62 | 1.4e-005 | 1Score > 26 indicates identity |
U | K.IQEIISASGAK.G | |
| 4133 |
607.8333 | 1213.6520 | 1213.6455 | 5.39 | 0 | 46 | 0.00061 | 1Score > 26 indicates identity |
U | K.HSFPQSSLIAK.I | |
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer4.rawScore > 25 indicates identity Score > 23 indicates homology |
|||||||||||
| 4159 |
709.8236 | 1417.6326 | 1417.6218 | 7.69 | 0 | 66 | 2.7e-006 | 1Score > 25 indicates identity Score > 23 indicates homology |
U | K.AMLQQDMTGYTK.G + 2 Oxidation (M) | |
| -46.3 | 0 | 6 | 2.6 | 2 | MALQQSDRPTVR + Oxidation (M); Deamidated (NQ) | ||||||
| -13.4 | 1 | 0 | 10 | 3 | VMKDMGMPQHTK + Oxidation (M) | ||||||
| 4185 |
840.9343 | 1679.8540 | 1679.8440 | 5.96 | 0 | 81 | 3.9e-008 | 1Score > 27 indicates identity Score > 20 indicates homology |
U | K.VIVDAYCTIPTVDSK.C | |
| 4254 |
704.0290 | 2109.0652 | 2109.0338 | 14.9 | 0 | 52 | 3.1e-005 | 1Score > 27 indicates identity Score > 19 indicates homology |
U | K.TIVLGAHQDSINLDSPSEGR.A + Deamidated (NQ) | |
| 4288 |
830.4249 | 2488.2529 | 2488.2180 | 14.0 | 0 | 39 | 0.00059 | 1Score > 26 indicates identity Score > 19 indicates homology |
U | K.GTTDAGKPESIGIITDNVDENLTK.F + Deamidated (NQ) | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | P20D2_ASPOR | 190 | Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ORYZ_ASPOR | 189 | Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U8Y3_ASPOR | 186 | Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UB64_ASPOR | 180 | SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2ULW8_ASPOR | 174 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EGLC_ASPOR | 161 | Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 138 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U2Z5_ASPOR | 107 | Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1 |
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